通过人乳头瘤病毒捕获技术对宫颈上皮内瘤变和正常宫颈上皮中人乳头瘤病毒DNA整合进行全基因组分析。
Genome-wide profiling of the human papillomavirus DNA integration in cervical intraepithelial neoplasia and normal cervical epithelium by HPV capture technology.
作者信息
Liu Ying, Zhang Chaoting, Gao Weijiao, Wang Limin, Pan Yaqi, Gao Yunong, Lu Zheming, Ke Yang
机构信息
Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Laboratory of Genetics, Peking University Cancer Hospital &Institute, No. 52 Fucheng Road, Haidian District, Beijing 100142, China.
Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Gynecologic Oncology, Peking University Cancer Hospital &Institute, No 52, Fucheng Road, Haidian District, Beijing 100142, China.
出版信息
Sci Rep. 2016 Oct 19;6:35427. doi: 10.1038/srep35427.
HPV integration plays an important role in cervical carcinogenesis. HPV genotypes and the exact integration sites were investigated using HPV capture technology combined with next generation sequencing in 166 women. Three, one and six integration sites were verified in 7 HPV-positive 'normal cervical epithelium', 6 HPV-positive CIN2 and 15 HPV-positive CIN 3 samples, respectively. Of the 10 integrations, one and nine were involved with HPV33 and HPV16, respectively. Our study accurately evaluated HPV integration level in CINs and normal cervical tissues using high-throughput viral integration detection method providing basic evidence for HPV integration-driven cervical carcinogenesis.
人乳头瘤病毒（HPV）整合在宫颈癌发生过程中起重要作用。采用HPV捕获技术结合新一代测序对166名女性的HPV基因型及确切整合位点进行了研究。在7例HPV阳性的“正常宫颈上皮”、6例HPV阳性的CIN2和15例HPV阳性的CIN3样本中，分别验证出3个、1个和6个整合位点。在这10个整合位点中，分别有1个和9个与HPV33和HPV16相关。我们的研究使用高通量病毒整合检测方法准确评估了CIN和正常宫颈组织中的HPV整合水平，为HPV整合驱动的宫颈癌发生提供了基础证据。
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