Lopes Novo Clara, Rugg-Gunn Peter
a Epigenetics Program , Babraham Institute , Cambridge , UK.
Nucleus. 2016 Sep 2;7(5):447-452. doi: 10.1080/19491034.2016.1248013.
Pluripotent cells are characterized by a globally open and accessible chromatin organization that is thought to contribute to cellular plasticity and developmental decision-making. We recently identified the pluripotency factor Nanog as a key regulator of this form of chromatin architecture in mouse embryonic stem cells. In particular, we demonstrated that the transcription factors Nanog and Sall1 co-dependently mediate the epigenetic state of pericentromeric heterochromatin to reinforce a more open and accessible organization in pluripotent cells. Here, we summarize our main findings and place the work into a broader context. We explore how heterochromatin domains could be targets of transcriptional networks in pluripotent cells and are coordinated with cell state. We propose this integration may be to balance the requirement for a dynamic and plastic chromatin organization in pluripotent cells, together with priming for a more restrictive nuclear compartmentalization that is triggered rapidly upon lineage commitment.
多能细胞的特征在于其全局开放且易于接近的染色质组织，这种组织被认为有助于细胞可塑性和发育决策。我们最近确定多能性因子Nanog是小鼠胚胎干细胞中这种染色质结构形式的关键调节因子。特别是，我们证明转录因子Nanog和Sall1共同依赖地介导着丝粒周围异染色质的表观遗传状态，以加强多能细胞中更开放且易于接近的组织。在这里，我们总结了我们的主要发现，并将这项工作置于更广泛的背景中。我们探讨了异染色质结构域如何成为多能细胞中转录网络的靶点，并与细胞状态相协调。我们提出这种整合可能是为了平衡多能细胞中对动态和可塑性染色质组织的需求，同时为在谱系定向时迅速触发的更具限制性的核区室化做好准备。
