Zheng Aijuan, Zhang Anrong, Chen Zhimin, Pirzado Shoaib Ahmed, Chang Wenhuan, Cai Huiyi, Bryden Wayne L, Liu Guohua
Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, No.12 Zhongguancun south street, Haidian district, Beijing, 100081, China.
School of Agriculture and Food Sciences, University of Queensland, Gatton, QLD, 4343, Australia.
J Anim Sci Biotechnol. 2021 Jul 13;12(1):90. doi: 10.1186/s40104-021-00591-1.
Immunological stress decreases feed intake, suppresses growth and induces economic losses. However, the underlying molecular mechanism remains unclear. Label-free liquid chromatography and mass spectrometry (LC-MS) proteomics techniques were employed to investigate effects of immune stress on the hepatic proteome changes of Arbor Acres broilers (Gallus Gallus domesticus) challenged with Escherichia coli lipopolysaccharide (LPS).
Proteomic analysis indicated that 111 proteins were differentially expressed in the liver of broiler chickens from the immune stress group. Of these, 28 proteins were down-regulated, and 83 proteins were up-regulated in the immune stress group. Enrichment analysis showed that immune stress upregulated the expression of hepatic proteins involved in defense function, amino acid catabolism, ion transport, wound healing, and hormone secretion. Furthermore, immune stress increased valine, leucine and isoleucine degradation pathways.
The data suggests that growth depression of broiler chickens induced by immune stress is triggered by hepatic proteome alterations, and provides a new insight into the mechanism by which immune challenge impairs poultry production.
免疫应激会降低采食量、抑制生长并造成经济损失。然而，其潜在的分子机制仍不清楚。采用无标记液相色谱和质谱（LC-MS）蛋白质组学技术，研究免疫应激对经大肠杆菌脂多糖（LPS）攻击的艾维茵肉鸡（家鸡）肝脏蛋白质组变化的影响。
蛋白质组分析表明，免疫应激组肉鸡肝脏中有111种蛋白质表达存在差异。其中，免疫应激组有28种蛋白质表达下调，83种蛋白质表达上调。富集分析表明，免疫应激上调了参与防御功能、氨基酸分解代谢、离子转运、伤口愈合和激素分泌的肝脏蛋白质的表达。此外，免疫应激增加了缬氨酸、亮氨酸和异亮氨酸的降解途径。
数据表明，免疫应激诱导的肉鸡生长抑制是由肝脏蛋白质组改变引发的，并为免疫挑战损害家禽生产的机制提供了新的见解。
