Abtahi Froushani Seyyed Meysam, Zarei Leila, Esmaeili Gouvarchin Ghaleh Hadi, Mansori Motlagh Bahman
Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran.
Solid Tumor Research Center, Urmia University of Medical Sciences, Urmia, Iran.
Avicenna J Phytomed. 2016 Sep-Oct;6(5):526-534.
Some evidence suggests that chronic uptake of estragole and methyl-eugenol, found in the essential oil of (tarragon), may be associated with an increased risk of hepato-carcinogenicity. The present study was conducted to investigate the immumodulatory and anti-inflammatory potentials of estragole and methyl-eugenol free extract of tarragon.
Aqueous, hydroalcoholic, methanol and hexane extracts of dried and milled tarragon was prepared and analyzed by GC-MS. The estragole and methyl-eugenol free extract was characterized and used for evaluation of immunity in NMRI mice after challenging with sheep red blood cells.
It was shown that the aqueous extract of tarragon was free from potentially harmful estragole or methyl-eugenol. Moreover, the immunomodulatory effect of the aqueous extract of tarragon (100 mg/kg for 21 consecutive days) was investigated. The extract significantly increased the level of anti-sheep red blood cells (SRBC (antibody and simultaneously decreased the level of cellular immunity in the treatment group. Moreover, tarragon caused a significant reduction in the production of pro-inflammatory IL-17 and IFN-γ in parallel with a reduction in the ratio of INF-γ to Il-10 or IL-17 to IL-10 in the splenocytes. In addition, the levels of the respiratory burst and nitric oxide production in peritoneal macrophages were significantly decreased. Additionally, the phagocytosis potential of macrophages was significantly increased in treated mice.
These data showed that the aqueous extract of tarragon may be used as a natural source to modulate the immune system, because it can inhibit pro-inflammatory cytokines and induce anti-inflammatory macrophages.
一些证据表明，龙蒿精油中含有的草蒿脑和甲基丁香酚的长期摄入可能与肝癌发生风险增加有关。本研究旨在调查无草蒿脑和甲基丁香酚的龙蒿提取物的免疫调节和抗炎潜力。
制备干燥并研磨后的龙蒿的水提取物、水醇提取物、甲醇提取物和己烷提取物，并通过气相色谱-质谱联用仪（GC-MS）进行分析。对无草蒿脑和甲基丁香酚的提取物进行表征，并用于评估经绵羊红细胞攻击后的NMRI小鼠的免疫力。
结果表明，龙蒿的水提取物不含潜在有害的草蒿脑或甲基丁香酚。此外，研究了龙蒿水提取物（连续21天给予100mg/kg）的免疫调节作用。该提取物显著提高了抗绵羊红细胞（SRBC）抗体水平，同时降低了治疗组的细胞免疫水平。此外，龙蒿使促炎细胞因子IL-17和IFN-γ的产生显著减少，同时脾细胞中INF-γ与Il-10或IL-17与IL-10的比例降低。此外，腹膜巨噬细胞的呼吸爆发水平和一氧化氮产生量显著降低。另外，处理过的小鼠中巨噬细胞的吞噬潜力显著增加。
这些数据表明，龙蒿水提取物可作为调节免疫系统的天然来源，因为它可以抑制促炎细胞因子并诱导抗炎巨噬细胞。
