Majdan Magdalena, Kiss Anna K, Hałasa Rafał, Granica Sebastian, Osińska Ewa, Czerwińska Monika E
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland.
Department of Bromatology, Faculty of Pharmacy, Medical University of Warsaw, Warsaw, Poland.
Front Pharmacol. 2020 Aug 13;11:947. doi: 10.3389/fphar.2020.00947. eCollection 2020.
The aim of the study was to characterize phytochemicals in an infusion of the aerial parts of tarragon L.) using ultra-high-performance liquid chromatography diode array detector electrospray ionisation tandem mass spectrometry UHPLC-DAD-ESI-MS/MS method, as well as an evaluation of its effects on mediators of the inflammation in an model of human neutrophils, and antimicrobial activity on selected pathogens. Flavonoids and caffeoylquinic acids were the main phenolic components of the extract of tarragon's aerial parts. The infusion was able to inhibit reactive oxygen species (ROS), interleukin 8 (IL-8), and tumour necrosis factor α (TNF-) production. The antimicrobial assay was performed with the use of nine strains of bacteria, both Gram-negative and Gram-positive. Three human pathogens, ATCC6538, ATCC14990, and MRSA (methicyllin-resistant ) ATCC43300, proved to be the most sensitive to tarragon infusion. Our study demonstrated the antiinflammatory and antimicrobial properties of tarragon L.), meaning the common spice may be a prospective source of health-promoting constituents.
本研究的目的是使用超高效液相色谱-二极管阵列检测器-电喷雾电离串联质谱法（UHPLC-DAD-ESI-MS/MS）对龙蒿地上部分浸液中的植物化学物质进行表征，并在人类中性粒细胞模型中评估其对炎症介质的影响以及对选定病原体的抗菌活性。黄酮类化合物和咖啡酰奎宁酸是龙蒿地上部分提取物的主要酚类成分。该浸液能够抑制活性氧（ROS）、白细胞介素8（IL-8）和肿瘤坏死因子α（TNF-α）的产生。使用9株革兰氏阴性和革兰氏阳性细菌进行了抗菌试验。三种人类病原体，即金黄色葡萄球菌ATCC6538、表皮葡萄球菌ATCC14990和耐甲氧西林金黄色葡萄球菌（MRSA）ATCC43300，被证明对龙蒿浸液最为敏感。我们的研究证明了龙蒿的抗炎和抗菌特性，这意味着这种常见的香料可能是促进健康成分的潜在来源。
