Wang Meng, Wang Guoqing, Zhang Ting, Fan Lihai, Tan Tianwei
National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, 15th, Beisanhuan East Road, Beijing, 100029, People's Republic of China.
Appl Microbiol Biotechnol. 2017 Jan;101(2):647-657. doi: 10.1007/s00253-016-7919-4. Epub 2016 Oct 19.
1,3-Propanediol (1,3-PDO) is a monomer for the synthesis of various polyesters. It is widely used in industries including cosmetics, solvents, and lubricants. Here, the multi-modular engineering was used to improve the concentration and tolerance of 1,3-PDO in Klebsiella pneumoniae. Firstly, the concentration of 1,3-PDO was increased by 25 %, while the concentrations of by-products were reduced considerably through one-step evolution which focused on the glycerol pathway. In addition, the 1,3-PDO tolerance was improved to 150 g L. Secondly, co-substrate transport system was regulated, and the 1,3-PDO concentration, yield, and productivity of the mutant were improved to 76.4 g L, 0.53 mol mol, and 2.55 g L h, respectively. Finally, NADH regeneration was introduced and the recombinant strain was successfully achieved with a high productivity of 2.69 g L h. The concentration and yield of 1,3-PDO were also improved to 86 g L and 0.59 mol mol. This strategy described here provides an approach of achieving a superior strain which is able to produce 1,3-PDO with high productivity and yield.
1,3-丙二醇（1,3-PDO）是合成各种聚酯的单体。它广泛应用于化妆品、溶剂和润滑剂等行业。在此，采用多模块工程来提高肺炎克雷伯菌中1,3-PDO的浓度和耐受性。首先，通过聚焦甘油途径的一步进化，1,3-PDO的浓度提高了25%，同时副产物的浓度大幅降低。此外，1,3-PDO耐受性提高到150 g/L。其次，调节共底物转运系统，突变体的1,3-PDO浓度、产量和生产率分别提高到76.4 g/L、0.53 mol/mol和2.55 g/(L·h)。最后，引入NADH再生，成功构建了重组菌株，其生产率高达2.69 g/(L·h)。1,3-PDO的浓度和产量也分别提高到86 g/L和0.59 mol/mol。本文所述策略提供了一种获得能够高产1,3-PDO的优良菌株的方法。
