Lee Jung Hun, Jung Moo-Young, Oh Min-Kyu
1Department of Chemical and Biological Engineering, Korea University, Seongbuk-gu, Seoul, 02841 Republic of Korea.
CJ Research Institute of Biotechnology, Suwon, Gyeonggi 16495 Republic of Korea.
Biotechnol Biofuels. 2018 Apr 9;11:104. doi: 10.1186/s13068-018-1100-5. eCollection 2018.
Glycerol is a major byproduct of the biodiesel industry and can be converted to 1,3-propanediol (1,3-PDO) by microorganisms through a two-step enzymatic reaction. The production of 1,3-PDO from glycerol using microorganisms is accompanied by formation of unwanted byproducts, including lactate and 2,3-butanediol, resulting in a low-conversion yield.
was metabolically engineered to produce high-molar yield of 1,3-PDO from glycerol. First, the pathway genes for byproduct formation were deleted in . . Then, glycerol assimilation pathways were eliminated and mannitol was co-fed to the medium. Finally, transcriptional regulation of the operon were genetically modified for enhancing 1,3-propanediol production. The batch fermentation of the engineered strain with co-feeding of a small amount of mannitol yielded 0.76 mol 1,3-PDO from 1 mol glycerol.
is useful microorganism for producing 1,3-PDO from glycerol. Implemented engineering in this study successfully improved 1,3-PDO production yield, which is significantly higher than those reported in previous studies.
甘油是生物柴油行业的主要副产物，可通过微生物经两步酶促反应转化为1,3 - 丙二醇（1,3 - PDO）。利用微生物从甘油生产1,3 - PDO的过程中会形成包括乳酸和2,3 - 丁二醇在内的不需要的副产物，导致转化率较低。
对（菌株名称缺失）进行代谢工程改造以从甘油中高产率生产1,3 - PDO。首先，在（菌株名称缺失）中删除了副产物形成的途径基因。然后，消除甘油同化途径并向培养基中共进料甘露醇。最后，对（操纵子名称缺失）操纵子进行转录调控以增强1,3 - 丙二醇的生产。工程菌株在少量甘露醇共进料的分批发酵中，从1摩尔甘油产生了0.76摩尔1,3 - PDO。
（菌株名称缺失）是用于从甘油生产1,3 - PDO的有用微生物。本研究中实施的工程成功提高了1,3 - PDO的产量，显著高于先前研究报道的产量。
