Mastrolia L, Gallo V P, Civinini A, Manelli H
Cell Mol Biol. 1989;35(3):247-53.
The presence of acetylcholinesterase (AChE) activity in the adrenal chromaffin cells of Necturus maculosus and Ambystoma maculatum (Amphibia, Urodela) has been demonstrated by cytochemical method at the electron microscope level. The enzymatic activity is localized in RER and perinuclear cisternae, on the plasma membrane and within the chromaffin vesicles, both in adrenaline (A) and noradrenaline (N) cells. Moreover N cells appear to be more reactive than A cells and Necturus more reactive than Ambystoma. The possible function of the AChE activity inside the vesicles is discussed as a mechanism of protons donor or as peptidasic activity acting on various peptides present in the vesicle.
利用细胞化学方法在电子显微镜水平上已证实，黄斑美西螈和斑泥螈（两栖纲，有尾目）的肾上腺嗜铬细胞中存在乙酰胆碱酯酶（AChE）活性。酶活性定位于粗面内质网和核周池、质膜以及嗜铬小泡内，在肾上腺素（A）细胞和去甲肾上腺素（N）细胞中均如此。此外，N细胞似乎比A细胞反应性更强，美西螈比泥螈反应性更强。文中讨论了小泡内AChE活性可能的功能，即作为质子供体机制或作为作用于小泡中各种肽的肽酶活性。
