Benedeczky I, Carmichael S W
Histochemistry. 1980;68(3):317-20. doi: 10.1007/BF00493260.
Histochemical evidence for magnesium-activated-ATPase activity of chromaffin granules in the hamster adrenal medulla is presented. A modified Wachstein-Meisel medium, using 40 mM magnesium, was employed. Not all chromaffin granules were reactive, but the reaction product was more noticable on noradrenaline granules than adrenaline granules. Presumtive exocytotic sites were very reactive. Small vesicles, elongated-granules, lysosomes, the plasma membrane, the endoplasmic reticulum, and mitochondria were also reactive.
本文提供了仓鼠肾上腺髓质嗜铬颗粒镁激活ATP酶活性的组织化学证据。采用了一种改良的Wachstein-Meisel培养基，其中镁离子浓度为40 mM。并非所有嗜铬颗粒都有反应，但去甲肾上腺素颗粒上的反应产物比肾上腺素颗粒上的更明显。推测的胞吐部位反应性很强。小泡、细长颗粒、溶酶体、质膜、内质网和线粒体也有反应。
