质子化多核苷酸结构 - 23. 聚（dG）.聚（dC）的酸碱滞后现象
Protonated polynucleotides structures - 23. The acid-base hysteresis of poly(dG).poly(dC).
作者信息
Thiele D, Marck C, Schneider C, Guschlbauer W
出版信息
Nucleic Acids Res. 1978 Jun;5(6):1997-2012. doi: 10.1093/nar/5.6.1997.
Abstract
The large hysteresis observed during the acid-base titration of poly(dG). poly (dC) was studied by CD and potentiometric scanning curves. Intermediate scanning loops as well as the equilibrium and metastable branches of the hysteresis loop have been determined. The potentiometric titrations showed, however, that the various complexes were not discrete entities, but were linked in "polycomplexes" as had been already suggested. This prevented a thermodynamic study of the system. The acid-base titration was further investigated as a function of ionic strength and temperature. The pK's showed considerably lower ionic strength dependence than observed for polyribonucleotide complexes. The thermal transitions permitted to establish the relative stabilities of the various complexes between pH 2.5 and pH 12.0.
摘要
通过圆二色光谱（CD）和电位扫描曲线研究了聚（dG）·聚（dC）酸碱滴定过程中观察到的大滞后现象。确定了中间扫描环以及滞后环的平衡和亚稳分支。然而，电位滴定表明，各种复合物并非离散实体，而是如之前所提出的那样以“多聚复合物”形式相连。这使得无法对该系统进行热力学研究。进一步研究了酸碱滴定随离子强度和温度的变化。与聚核糖核苷酸复合物相比，pK值对离子强度的依赖性要低得多。热转变有助于确定pH 2.5至pH 12.0之间各种复合物的相对稳定性。
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