Misra Biswapriya B, Yin Zepeng, Geng Sisi, de Armas Evaldo, Chen Sixue
Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32610, USA.
Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China.
Sci Rep. 2016 Oct 20;6:35778. doi: 10.1038/srep35778.
Global CO level presently recorded at 400 ppm is expected to reach 550 ppm in 2050, an increment likely to impact plant growth and productivity. Using targeted LC-MS and GC-MS platforms we quantified 229 and 29 metabolites, respectively in a time-course study to reveal short-term responses to different concentrations (1, 3, and 10 mM) of bicarbonate (HCO) under light and dark conditions. Results indicate that HCO treatment responsive metabolomic changes depend on the HCO concentration, time of treatment, and light/dark. Interestingly, 3 mM HCO concentration treatment induced more significantly changed metabolites than either lower or higher concentrations used. Flavonoid biosynthesis and glutathione metabolism were common to both light and dark-mediated responses in addition to showing concentration-dependent changes. Our metabolomics results provide insights into short-term plant cellular responses to elevated HCO concentrations as a result of ambient increases in CO under light and dark.
目前记录的全球一氧化碳水平为400ppm，预计到2050年将达到550ppm，这种增加可能会影响植物生长和生产力。在一项时间进程研究中，我们分别使用靶向液相色谱-质谱联用仪（LC-MS）和气相色谱-质谱联用仪（GC-MS）平台对229种和29种代谢物进行了定量分析，以揭示在光照和黑暗条件下对不同浓度（1、3和10mM）碳酸氢盐（HCO）的短期反应。结果表明，HCO处理引起的代谢组学变化取决于HCO浓度、处理时间以及光照/黑暗条件。有趣的是，3mM HCO浓度处理诱导的代谢物变化比使用的较低或较高浓度更为显著。除了显示浓度依赖性变化外，类黄酮生物合成和谷胱甘肽代谢在光照和黑暗介导的反应中均很常见。我们的代谢组学结果为光照和黑暗条件下由于环境中CO增加导致HCO浓度升高时植物细胞的短期反应提供了见解。
