Savino Francesco, Quartieri Andrea, De Marco Angela, Garro Maria, Amaretti Alberto, Raimondi Stefano, Simone Marta, Rossi Maddalena
Department of Pediatrics, Regina Margherita Children's Hospital, Città della Salute e della Scienza di Torino, Torino, Italy.
Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
Acta Paediatr. 2017 Apr;106(4):573-578. doi: 10.1111/apa.13642. Epub 2016 Nov 15.
This study compared the faecal microbial composition of formula-fed infants who did and did not have colic.
Faecal samples from formula-fed infants under 16 weeks of age with (n = 38) and without (n = 39) colic were collected at Department of Pediatrics in Turin, Italy, between February 2014 and October 2015. The pH and faecal ammonia were determined and total bacteria, bifidobacteria, lactic acid bacteria and coliforms were quantified by fluorescent in situ hybridisation (FISH).
Faecal ammonia was significantly higher in the colicky infants than in the controls (483 vs. 216 μg/g, p < 0.05). The FISH counts of total bacteria were lower in colicky infants (1.8E10 ± 1.5E10) than in the controls (3.4E10 ± 3.0E10) (p < 0.05). The relative abundance of coliform bacteria was significantly higher in colicky infants (p < 0.05). No differences were observed for the bifidobacteria and lactic acid bacteria counts between the two groups.
Our comparison of formula-fed infants with and without colic revealed significant differences in total bacteria, Enterobacteriaceae and faecal ammonia. This study provides the stimulus for further studies of the gut microbiome, using new methods of analysis such as 16S metagenomics sequencing in order to lead to more tailored dietary approaches.
本研究比较了患腹绞痛和未患腹绞痛的配方奶喂养婴儿的粪便微生物组成。
2014年2月至2015年10月期间，在意大利都灵的儿科收集了16周龄以下患腹绞痛（n = 38）和未患腹绞痛（n = 39）的配方奶喂养婴儿的粪便样本。测定了pH值和粪便氨含量，并通过荧光原位杂交（FISH）对总细菌、双歧杆菌、乳酸菌和大肠菌群进行了定量分析。
腹绞痛婴儿的粪便氨含量显著高于对照组（483 vs. 216 μg/g，p < 0.05）。腹绞痛婴儿的总细菌FISH计数低于对照组（1.8E10 ± 1.5E10 vs. 3.4E10 ± 3.0E10）（p < 0.05）。腹绞痛婴儿中大肠菌群的相对丰度显著更高（p < 0.05）。两组之间双歧杆菌和乳酸菌计数未观察到差异。
我们对患腹绞痛和未患腹绞痛的配方奶喂养婴儿的比较显示，总细菌、肠杆菌科和粪便氨存在显著差异。本研究为进一步研究肠道微生物组提供了动力，可采用16S宏基因组测序等新的分析方法，以实现更具针对性的饮食方法。
