富血小板纤维蛋白对周围神经再生的影响。

Effect of Platelet-Rich Fibrin on Peripheral Nerve Regeneration.

作者信息

Şenses Fatma, Önder Mustafa E, Koçyiğit Ismail D, Kul Oğuz, Aydin Gülümser, Inal Elem, Atil Fethi, Tekin Umut

机构信息

*Atasehir ADSM, Barbaros Mah, Istanbul†Department of OMFS, Faculty of Dentistry, Kirikkale University‡Department of Pathology, Faculty of Veterinary, University of Kirikkale, Kirikkale||Department of Physical Medicine and Rehabilitation, Faculty of Medicine, University of Yildirim Beyazit, Ankara¶Adil Mah. Polonez Cad. Sinpas Liva Turkuaz, Istanbul, Turkey.

出版信息

J Craniofac Surg. 2016 Oct;27(7):1759-1764. doi: 10.1097/SCS.0000000000003066.

DOI:10.1097/SCS.0000000000003066

PMID:27763975

Abstract

PURPOSE

This study aimed to evaluate the effect of platelet-rich fibrin (PRF) on peripheral nerve regeneration on the sciatic nerve of rats by using functional, histopathologic, and electrophysiologic analyses.

MATERIALS AND METHODS

Thirty female Wistar rats were divided randomly into 3 experimental groups. In group 1 (G1), which was the control group, the sciatic nerve was transected and sutured (n = 10). In group 2 (G2), the sciatic nerve was transected, sutured, and then covered with PRF as a membrane (n = 10). In group 3 (G3), the sciatic nerve was transected, sutured by leaving a 5-mm gap, and then covered by PRF as a nerve guide (n = 10). Functional, histopathologic, and electrophysiologic analyses were performed.

RESULTS

The total histopathologic semiquantitative score was significantly higher in G1 compared to G2 and G3 (P < 0.05). Myelin thickness and capillaries were significantly lower in G3 compared to G1 (P < 0.05). There was no statistically significant difference between the groups with regard to the functional and electrophysiologic results.

CONCLUSION

The study results suggest that PRF decreases functional recovery in sciatic nerve injury. Further studies are required to determine the efficacy of PRF on peripheral nerve regeneration.

摘要

目的

本研究旨在通过功能、组织病理学和电生理学分析，评估富血小板纤维蛋白（PRF）对大鼠坐骨神经周围神经再生的影响。

材料与方法

30只雌性Wistar大鼠随机分为3个实验组。第1组（G1）为对照组，切断并缝合坐骨神经（n = 10）。第2组（G2）切断并缝合坐骨神经，然后用PRF作为膜覆盖（n = 10）。第3组（G3）切断坐骨神经，留5 mm间隙缝合，然后用PRF作为神经导管覆盖（n = 10）。进行功能、组织病理学和电生理学分析。