Rady Dina, Mubarak Rabab, Abdel Moneim Rehab A
Department of Oral Biology, Faculty of Dentistry, Cairo University, Cairo, 11553, Egypt.
F1000Res. 2018 Sep 28;7:1573. doi: 10.12688/f1000research.15985.1. eCollection 2018.
Various techniques for tissue engineering have been introduced to aid the regeneration of defective or lost bone tissue. The aim of this study was to compare the bone-forming potential of bone marrow mesenchymal stem cells (BM-MSCs) and platelet-rich fibrin (PRF) on induced bone defects in rats' tibiae. In total, one defect of 3-mm diameter was created in each tibia of 36 Wistar male rats. There were two groups: group A, left tibia bone defects that received PRF; and group B, right tibia bone defects of the same animal that received BM-MSCs loaded on a chitosan scaffold. Subsequently, Scanning electron microscope/energy-dispersive X-ray (SEM/EDX) analyses was performed at 3 and 10 days, and 3 weeks post‑implantation and following euthanasia; (n=12). The EDX analysis performed for each group and time point revealed a significant increase in the mean calcium and phosphorous weight percentage in the BM-MSC-treated group relative to the PRF-treated group at all-time intervals (P < 0.05). Moreover, the mean calcium and phosphorus weight percentage increased as time progressed since the surgical intervention in the PRF-treated and BM-MSCs groups (P < 0.05). In the present study, both BM-MSCs and PRF were capable of healing osseous defects induced in a rat tibial model. Yet, BM-MSCs promoted more adequate healing, with higher mean calcium and phosphorous weight percentages than PRF at all-time points, and showed greater integration into the surrounding tissues than PRF.
已经引入了各种组织工程技术来辅助有缺陷或缺失的骨组织的再生。本研究的目的是比较骨髓间充质干细胞（BM-MSCs）和富血小板纤维蛋白（PRF）对大鼠胫骨诱导性骨缺损的成骨潜力。总共在36只雄性Wistar大鼠的每只胫骨上制造一个直径3毫米的缺损。分为两组：A组，左胫骨骨缺损接受PRF；B组，同一只动物的右胫骨骨缺损接受负载于壳聚糖支架上的BM-MSCs。随后，在植入后3天、10天、3周以及安乐死后进行扫描电子显微镜/能量色散X射线（SEM/EDX）分析；（n = 12）。对每组和每个时间点进行的EDX分析显示，在所有时间间隔内，BM-MSC治疗组的钙和磷平均重量百分比相对于PRF治疗组均显著增加（P < 0.05）。此外，自手术干预以来，PRF治疗组和BM-MSCs组的钙和磷平均重量百分比随时间推移而增加（P < 0.05）。在本研究中，BM-MSCs和PRF都能够治愈大鼠胫骨模型中诱导的骨缺损。然而，BM-MSCs促进了更充分的愈合，在所有时间点的钙和磷平均重量百分比均高于PRF，并且比PRF更能融入周围组织。
