Laboratório de Retroviroses, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil.
Setor de Medicina Veterinária Preventiva, Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
BMC Vet Res. 2016 Oct 20;12(1):238. doi: 10.1186/s12917-016-0845-y.
The presence of lymphoma in buffaloes was first reported in India in the 1960s. The disease is similar to Enzootic Bovine Leucosis (EBL) caused by Bovine leukemia virus (BLV) in cattle; however, according to our results and those of other studies, the etiology of these lymphomas in buffalo do not appear to be associated with BLV. The objectives of this study are to describe four cases of the disease in buffaloes belonging to the same herd in the Amazon region of Brazil and to perform a clinical-anatomopathological, immunohistochemical, and etiological study of the lymphomas.
Over a period of ten years, four buffaloes were observed presenting progressive weight loss, swelling of peripheral lymph nodes, and nodules in the subcutaneous tissue. Upon necropsy, whitish-colored tumor masses were observed in the form of nodules in the subcutaneous tissue, along with miliary nodules on the serosal surfaces of abdominal and thoracic organs and tumors in lymph nodes and other organs. Neoplastic lymphocyte proliferation was observed through histopathology. An immunohistochemical study revealed that the neoplasias were formed by proliferation of predominantly B lymphocytes. The presence of BLV genome was not detected in the lymphomas when using the real-time PCR technique, nor was it detected through immunohistochemical staining using monoclonal antibodies against two viral proteins. Bovine herpesvirus 6 was not detected in the tumors. However, Bovine immunodeficiency virus (BIV) was detected in samples of lymphoma and in the lymph nodes and kidneys of one of the animals.
The occurrence of lymphoma in buffaloes is reported for the first time in Brazil and is characterized by B-cell multicentric lymphoma. The etiology of the disease does not appear to be associated with BLV; however, the detection of BIV in samples of lymphoma from one sick animal deserves further study, considering the oncogenic potential of this virus.
20 世纪 60 年代，印度首次报道了水牛淋巴瘤的存在。这种疾病与牛白血病病毒（BLV）引起的牛地方性白血病（EBL）相似；然而，根据我们和其他研究的结果，这些水牛淋巴瘤的病因似乎与 BLV 无关。本研究的目的是描述巴西亚马逊地区同一牛群中 4 例水牛的疾病，并对其进行临床解剖病理学、免疫组织化学和病因学研究。
在十年的时间里，观察到 4 头水牛逐渐消瘦，外周淋巴结肿大，皮下组织出现结节。剖检时，在皮下组织中观察到灰白色的肿瘤块，以及腹部和胸部器官的浆膜表面的粟粒状结节和淋巴结及其他器官的肿瘤。组织病理学观察到淋巴细胞的肿瘤性增殖。免疫组织化学研究显示，肿瘤主要由 B 淋巴细胞增殖形成。实时 PCR 技术未检测到淋巴瘤中 BLV 基因组的存在，也未通过针对两种病毒蛋白的单克隆抗体的免疫组织化学染色检测到。肿瘤中未检测到牛疱疹病毒 6，但在一个动物的淋巴瘤样本和淋巴结及肾脏中检测到牛免疫缺陷病毒（BIV）。
首次在巴西报道了水牛的淋巴瘤，其特征为 B 细胞多中心性淋巴瘤。该疾病的病因似乎与 BLV 无关；然而，在一只患病动物的淋巴瘤样本中检测到 BIV，值得进一步研究，因为该病毒具有致癌潜力。
