Chen M M, Tan Y, Tang Z Z, Lin M, Zhou K J, He W T, Yang Y P, Wang J
Institute of Emerging Infectious Diseases Prevention and Control, Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530028, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2016 Oct 10;37(10):1350-1355. doi: 10.3760/cma.j.issn.0254-6450.2016.10.007.
To understand the epidemiological characteristics and viral sources of dengue fever outbreak in Guangxi Zhuang Autonomous Region (Guangxi) in 2014. A combined analysis of epidemiological characteristics and genetic characteristics were performed in this study. The time, population and area distributions of the cases were analyzed. Serum samples were collected from dengue fever cases to detect NS1 antigen by using commercial ELISA kits according to the guideline of the manufacture. RT-PCR assay was conducted to detect dengue virus in NS1 positive samples. Phylogenetic tree based on E gene sequence of dengue virus were further analyzed. During September-December 2014, an outbreak of dengue fever caused by dengue virus type 1 and 2 occurred in Guangxi, a total of 854 cases were reported without death, including 712 laboratory confirmed cases and 142 clinical diagnosed cases, in which 79.63 (680/854) occurred during 22 September-21 October 2014. All the cases had typical dengue fever symptoms. Most cases occurred in Nanning and Wuzhou, in which 83.61 (714/854) were in age group 15-59 years; 46.60 (398/854) were staff or people engaged in commercial service. A total 526 serum samples were tested for dengue virus serotype by RT-PCR assay. Among 414 positive samples, 345 were positive for dengue virus type 1 (DENV-1) and 69 were positive for dengue virus type 2 (DENV-2), no DENV-3 and DENV-4 were detected. The results of phylogenetic analysis of E gene sequence indicated that the sequences of 99.12(113/114) of DENV-1 strains in Nanning in China shared 100.00 homology with the isolate (SG EHI D1/529Y13) from Singapore in 2013, which belonged to the genotype Ⅰ; All the DENV-2 isolates from Wuzhou shared 99.80 homology with the isolate (D14005) from Guangdong province, which belonged to genotype Cosmopolitan. The outbreak was caused by DENV-1 from Singapore and DENV-2 from Guangdong province in China. It is necessary to strengthen the surveillance and early warning for imported dengue fever, conduct vector control and improve the diagnosis of suspected dengue fever cases for the effective control of dengue fever outbreak.
为了解2014年广西壮族自治区登革热疫情的流行病学特征及病毒来源。本研究对流行病学特征和基因特征进行了综合分析。分析了病例的时间、人群和地区分布。按照制造商指南，使用商用酶联免疫吸附测定试剂盒从登革热病例中采集血清样本以检测NS1抗原。对NS1阳性样本进行逆转录聚合酶链反应检测以检测登革病毒。进一步分析基于登革病毒E基因序列的系统发育树。2014年9月至12月，广西发生了由1型和2型登革病毒引起的登革热疫情，共报告854例，无死亡病例，其中实验室确诊病例712例，临床诊断病例142例，其中79.63%（680/854）的病例发生在2014年9月22日至10月21日期间。所有病例均有典型的登革热症状。大多数病例发生在南宁和梧州，其中83.61%（714/854）为15 - 59岁年龄组；46.60%（398/854）为工作人员或从事商业服务的人员。通过逆转录聚合酶链反应检测共526份血清样本的登革病毒血清型。在414份阳性样本中，345份为1型登革病毒（DENV - 1）阳性，69份为2型登革病毒（DENV - 2）阳性，未检测到DENV - 3和DENV - 4。E基因序列的系统发育分析结果表明，中国南宁99.12%（113/114）的DENV - 1毒株序列与2013年来自新加坡的分离株（SG EHI D1/529Y13）具有100.00%的同源性，属于Ⅰ基因型；梧州所有的DENV - 2分离株与来自中国广东省的分离株（D14005）具有99.80%的同源性，属于泛基因型。此次疫情由来自新加坡的DENV - 1和来自中国广东省的DENV - 2引起。有必要加强对输入性登革热的监测和预警，开展媒介控制并改善疑似登革热病例的诊断，以有效控制登革热疫情爆发情况。
