Molecular Biology Laboratory, Faculty of Veterinary Medicine, University of Khartoum, P.O. Box 32, Khartoum North, Sudan.
Department of Internal Medicine and Microbiology, Faculty of Medicine, University of Kassala, El-Gadarif, Sudan.
Virol J. 2020 Jul 30;17(1):118. doi: 10.1186/s12985-020-01389-9.
Dengue fever (DF) is an arthropod-borne disease caused by dengue virus (DENV). DENV is a member of the genus Flavivirus in the family Flaviviridae. Recently, DENV has been reported as an important emerging infectious viral pathogen in Sudan. Multiple outbreaks and sporadic cases of DF have been frequently reported in the eastern region of Sudan. The present study was conducted to confirm DENV outbreak in Kassala State, eastern Sudan, 2019, and to provide some information on the molecular characterization of the DENV isolate associated with the disease outbreak.
A hundred serum samples were collected during the outbreak from residents of Kassala State, Sudan, 2019. ELISA was used to detect DENV non structural protein NS1 (DENV-NS1) in acute phase sera sampled during the disease outbreak. RT-PCR assays were used to amplify a fragment of the capsid/pre-membrane region (CprM) of the viral polyprotein gene. The PCR products of the amplified CprM region of the viral polyprotein gene were purified and partial sequences were generated and used to confirm the specificity of DENV sequences and to identify the virus serotype. Phylogenetic tree was constructed to determine the genotype of DENV associated with the outbreak.
Using DENV-NS1 ELISA assay, DENV infection was confirmed in 23% sampled sera. The detection of DENV RNA was made possible using group-specific RT-PCR assay. The virus was serotyped as DENV serotype 3 (DENV-3) using DENV serotype-specific RT-PCR assay. Phylogenetic analysis of the partial CprM sequences of the viral polyprotein gene indicates that the virus belonged to genotype III of DENV-3.
The scientific data presented in this investigation confirmed that genotype III of DENV-3 was associated with the disease outbreak in eastern Sudan, 2019. The study represents the first report on molecular characterization of DENV-3 in Sudan.
登革热（DF）是一种由登革病毒（DENV）引起的虫媒病。DENV 是黄病毒科黄病毒属的成员。最近，DENV 已被报道为苏丹的一种重要新兴传染性病毒病原体。在苏丹东部地区，经常报告 DF 的多次暴发和散发病例。本研究旨在确认 2019 年在苏丹东部卡萨拉州发生的 DENV 暴发，并提供与疾病暴发相关的 DENV 分离株的分子特征的一些信息。
在 2019 年暴发期间，从苏丹卡萨拉州的居民中采集了 100 份血清样本。使用 ELISA 检测急性疾病暴发期间采集的血清样本中的 DENV 非结构蛋白 NS1（DENV-NS1）。使用 RT-PCR 检测病毒衣壳/前膜区（CprM）的聚合酶基因的扩增片段。对扩增的病毒多蛋白基因的 CprM 区的 PCR 产物进行纯化，并生成部分序列，以确认 DENV 序列的特异性并鉴定病毒血清型。构建系统发育树以确定与暴发相关的 DENV 基因型。
使用 DENV-NS1 ELISA 检测试剂盒，在 23%的采样血清中证实了 DENV 感染。使用组特异性 RT-PCR 检测试剂盒可检测到 DENV RNA。使用 DENV 血清型特异性 RT-PCR 检测试剂盒将病毒血清型鉴定为 DENV 血清型 3（DENV-3）。病毒多蛋白基因的部分 CprM 序列的系统发育分析表明，该病毒属于 DENV-3 的基因型 III。
本研究中的科学数据证实，基因型 III 的 DENV-3 与 2019 年苏丹东部的疾病暴发有关。该研究代表了苏丹对 DENV-3 进行分子特征描述的首次报告。
Zotero 插件