Kostanyan Artak E
Kurnakov Institute of General & Inorganic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 31, Moscow 119991, Russia.
J Chromatogr A. 2016 Nov 4;1471:94-101. doi: 10.1016/j.chroma.2016.10.012. Epub 2016 Oct 14.
The closed-loop recycling counter-current chromatography (CLR CCC) is performed in several consecutive separation stages. First, the loop is opened, and within a specified time the solution of solutes in the mobile phase is continuously fed to the column. After the solutes loading is finished, the loop is closed, and the first separation stage starts. After a certain number of cycles the first fraction of solutes is eluted, the loop is closed, and the second separation stage starts, and so on. In this study, simple equations are presented allowing the simulation of such separation processes. These equations can help to select a suitable compromise between the productivity and the resolution in the preparative and production CLR CCC separations. It is shown that the sample loading time about 20%-30% of the mean residence time is quite acceptable from the practical point of view: proper selection of the loading time and the recycling line length can allow increasing the productivity by an order of magnitude ensuring, a desirable separation.
闭环循环逆流色谱法（CLR CCC）在几个连续的分离阶段进行。首先，打开环路，在规定时间内将溶质在流动相中的溶液连续进料到柱中。溶质加载完成后，关闭环路，第一个分离阶段开始。经过一定数量的循环后，洗脱第一部分溶质，关闭环路，第二个分离阶段开始，依此类推。在本研究中，给出了简单的方程，用于模拟这种分离过程。这些方程有助于在制备和生产CLR CCC分离中，在生产率和分辨率之间选择合适的折衷方案。结果表明，从实际角度来看，样品加载时间约为平均停留时间的20%-30%是相当可以接受的：适当选择加载时间和循环管线长度可以使生产率提高一个数量级，确保实现理想的分离。
