Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 31, 119991 Moscow, Russia.
Molecules. 2021 Oct 29;26(21):6561. doi: 10.3390/molecules26216561.
We previously reported on a new counter-current chromatography (CCC) operating mode called closed-loop recycling dual-mode counter-current chromatography (CLR DM CCC), which incorporates the advantages of closed-loop recycling (CLR) and dual-mode (DM) counter-current chromatography and includes sequential separation of compounds in the closed-loop recycling mode with the mobile -phase and in the inverted-phase counter-current mode with the mobile -phase. The theoretical analysis of several implementations of this separation method was carried out under impulse sample injection conditions. This study is dedicated to the further development of CLR DM CCC theory applied to preparative and industrial separations, where high-throughput operation is required. Large sample volumes can be loaded via continuous loading within a specified time. To simulate CLR DM CCC separations with specified sample loading durations, equations are developed and presented in "Mathcad" software.
我们之前报道了一种新的逆流色谱（CCC）操作模式，称为闭路循环再循环双模式逆流色谱（CLR DM CCC），它结合了闭路循环（CLR）和双模式（DM）逆流色谱的优点，并包括在闭路循环再循环模式下顺序分离化合物与流动相和反相逆流模式下的流动相。在脉冲进样条件下，对该分离方法的几种实现进行了理论分析。本研究致力于进一步发展应用于制备和工业分离的 CLR DM CCC 理论，其中需要高通量操作。可以通过在指定时间内连续加载来加载大的样品体积。为了模拟具有指定样品加载持续时间的 CLR DM CCC 分离，开发了方程并在“Mathcad”软件中呈现。
