Li W K
Biological Oceanography Division, Bedford Institute of Oceanography, Dartmouth, Nova Scotia, Canada.
Cytometry. 1989 Sep;10(5):564-79. doi: 10.1002/cyto.990100512.
Shipboard analysis of marine ultraphytoplankton by flow cytometry is a powerful method to classify these cells according to in vivo fluorescence characteristics and size. At present, this ataxonomic-allometric approach allows recognition of phycoerythrin-containing cyanobacteria, cryptomonads, very small red-fluorescing cells (presumably prochlorophytes), and eukaryotic algae of various sizes in many open ocean samples. The speed at which flow cytometric analysis can be performed on freshly collected samples permits a high degree of sampling resolution in both space and time. A flow cytometric view is presented of the vertical distribution of ultraphytoplankton at various sites in the north Atlantic and of experiments wherein phytoplankton were incubated in an artificial light gradient and under simulated in situ conditions.
通过流式细胞术对海洋超微型浮游植物进行船上分析，是一种根据体内荧光特征和大小对这些细胞进行分类的强大方法。目前，这种非分类学的异速生长方法能够识别许多开阔海洋样本中含藻红蛋白的蓝细菌、隐藻、非常小的红色荧光细胞（可能是原绿球藻）以及各种大小的真核藻类。对新鲜采集样本进行流式细胞术分析的速度，使得在空间和时间上都能实现高度的采样分辨率。本文展示了北大西洋不同地点超微型浮游植物的垂直分布情况，以及在人工光梯度和模拟原位条件下对浮游植物进行培养的实验的流式细胞术观察结果。
