Campbell J W, Yentsch C M
Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, Maine 04575.
Cytometry. 1989 Sep;10(5):596-604. doi: 10.1002/cyto.990100515.
In part I of this series of articles, a framework was presented for interpreting histograms of volume or fluorescence as measured by a flow cytometer on homogeneous phytoplankton populations. In this paper, the analytical framework is applied to flow cytometric histograms from laboratory experiments involving clonal phytoplankton cultures. The density function derived in part I was modified to include a third parameter representing a linear shift of the origin. This modified density function was fitted to chlorophyll fluorescence histograms for populations believed to be asynchronous (grown in continuous light) and also to histograms from populations grown on a 14:10 (h:h) light/dark cycle. Near-synchronous subpopulations sorted from an asynchronous population were also analyzed. In populations in which underlying assumptions (asynchronous divisions, constant growth) are valid, curve fits provide estimates of the inherent variability among cells at age 0. The implication of fitting the density function to populations in which these assumptions are not valid is discussed.
在本系列文章的第一部分，提出了一个框架，用于解释通过流式细胞仪测量的均匀浮游植物群体的体积或荧光直方图。在本文中，该分析框架应用于涉及克隆浮游植物培养物的实验室实验的流式细胞术直方图。第一部分中推导的密度函数进行了修改，以纳入代表原点线性位移的第三个参数。这个修改后的密度函数被拟合到被认为是异步的（在连续光照下生长）群体的叶绿素荧光直方图，以及在14:10（小时：小时）光/暗周期下生长的群体的直方图。还分析了从异步群体中分选出来的近同步亚群体。在基本假设（异步分裂、恒定生长）有效的群体中，曲线拟合提供了0龄细胞间固有变异性的估计。讨论了将密度函数拟合到这些假设无效的群体中的意义。
