Wu Li, Li Jie, Xu Hui Li, Xu Bo, Tong Xian Hong, Kwak-Kim Joanne, Liu Yu Sheng
Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei, Anhui, China.
Reproductive Medicine, Department of Obstetrics and Gynecology, Chicago Medical School at Rosalind Franklin University of Medicine and Science, Vernon Hills, IL, USA.
Am J Reprod Immunol. 2016 Dec;76(6):454-464. doi: 10.1111/aji.12588. Epub 2016 Oct 21.
We aim to investigate a possible role of IL-7/IL-7R signaling pathway in recurrent pregnancy losses (RPL).
Using the abortion-prone (AP) and non-abortion-prone (NP) mice model, fetal resorption rates (FRR), Th17 and Treg cells-related factors, and the effect of IL-7 and IL-7R antagonist were investigated by flow cytometry, quantitative real-time PCR, and immunohistochemistry. IL-7 and IL-7R expressions in human decidua were investigated by immunohistochemistry.
In the AP mice, IL-7R antagonist treatment significantly decreased FRR by downregulating Th17 and upregulating Treg-related factors. When the NP mice were treated with IL-7, FRR was significantly increased by upregulating Th17 and downregulating Treg-related factors. In decidual stromal cells of women with RPL, increased IL-7 and decreased IL-7R expressions were present when compared to normal controls.
IL-7/IL-7R signaling pathway plays a possible role in RPL by upregulating Th17 immunity, meanwhile downregulating Treg immunity. Regulation of IL-7/IL-7R may be a new therapeutic strategy for RPL.
我们旨在研究白细胞介素-7/白细胞介素-7受体（IL-7/IL-7R）信号通路在复发性流产（RPL）中的可能作用。
使用易流产（AP）和不易流产（NP）小鼠模型，通过流式细胞术、定量实时聚合酶链反应和免疫组织化学研究胎儿吸收率（FRR）、Th17和调节性T细胞（Treg）相关因子，以及IL-7和IL-7R拮抗剂的作用。通过免疫组织化学研究人蜕膜中IL-7和IL-7R的表达。
在AP小鼠中，IL-7R拮抗剂治疗通过下调Th17并上调Treg相关因子，显著降低了FRR。当用IL-7处理NP小鼠时，通过上调Th17并下调Treg相关因子，FRR显著增加。与正常对照相比，RPL女性的蜕膜基质细胞中IL-7表达增加，IL-7R表达降低。
IL-7/IL-7R信号通路可能通过上调Th17免疫，同时下调Treg免疫在RPL中发挥作用。调节IL-7/IL-7R可能是RPL的一种新治疗策略。
