Leroy Félix, Lamotte d'Incamps Boris
Centre National de la Recherche Scientifique (UMR 8119), Centre de Neurophysique, Physiologie et Pathologie, Université Paris Descartes;
Centre National de la Recherche Scientifique (UMR 8119), Centre de Neurophysique, Physiologie et Pathologie, Université Paris Descartes.
J Vis Exp. 2016 Oct 13(116):54525. doi: 10.3791/54525.
Electrophysiological recordings from spinal cord slices have proven to be a valuable technique to investigate a wide range of questions, from cellular to network properties. We show how to prepare viable oblique slices of the spinal cord of young mice (P2 - P11). In this preparation, the motoneurons retain their axons coming out from the ventral roots of the spinal cord. Stimulation of these axons elicits back-propagating action potentials invading the motoneuron somas and exciting the motoneuron collaterals within the spinal cord. Recording of antidromic action potentials is an immediate, definitive and elegant way to characterize motoneuron identity, which surpasses other identification methods. Furthermore, stimulating the motoneuron collaterals is a simple and reliable way to excite the collateral targets of the motoneurons within the spinal cord, such as other motoneurons or Renshaw cells. In this protocol, we present antidromic recordings from the motoneuron somas as well as Renshaw cell excitation, resulting from ventral root stimulation.
脊髓切片的电生理记录已被证明是一种有价值的技术，可用于研究从细胞特性到网络特性等广泛问题。我们展示了如何制备幼年小鼠（P2 - P11）脊髓的可行斜切片。在这种制备方法中，运动神经元保留了从脊髓腹根发出的轴突。刺激这些轴突会引发反向传播动作电位，侵入运动神经元胞体并兴奋脊髓内的运动神经元侧支。记录逆向动作电位是表征运动神经元身份的一种直接、明确且精妙的方法，它优于其他识别方法。此外，刺激运动神经元侧支是兴奋脊髓内运动神经元侧支靶标（如其他运动神经元或闰绍细胞）的一种简单可靠的方法。在本方案中，我们展示了来自运动神经元胞体的逆向记录以及腹根刺激引起的闰绍细胞兴奋。
