Wang Fei, Wu Dinglan, He Fengrong, Fu Housheng, Xu Jianbing, Chen Jianxiang, Zhang Shufang, Wang Weifu
Department of Urology, The Hospital of Hainan Province, Haikou - China.
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong - China.
Tumori. 2017 Nov 23;103(6):537-542. doi: 10.5301/tj.5000569. Epub 2016 Oct 21.
Cofilin 1 is a type of cytoskeletal protein. The overexpression of this gene has been regarded to hold a special relationship with the development and progress of some cancers. However, the detailed position of Cofilin 1 in human bladder cancer has not been investigated intensively.
In this study, we mainly explored the relationship between human bladder cancer and the expression of Cofilin 1. The expression of Cofilin 1 in bladder cancer tissues and paracancerous tissues of patients was evaluated with quantitative polymerase chain reaction, Western blot, and immunohistochemical staining. Downregulation of Cofilin 1 expression model was established with siRNA in human RT4 bladder cancer cell line, and the changing cell viability was analyzed to determine the role of Cofilin 1 in human bladder cancer.
Our results showed that the expression of Cofilin 1 was much higher in both RNA level and protein level in human bladder cancer tissues than paracancerous tissues for 3 patients. Downregulation of Cofilin 1 expression could inhibit cell proliferation, cell migration, cell adhesion, and colony formation ability, and increase the percentage of cell apoptosis in RT4 cells.
Our study indicates that Cofilin 1 holds an important position in the development and progression of human bladder cancer, and this gene might become a novel target in the diagnosis and treatment of human bladder cancer.
丝切蛋白1是一种细胞骨架蛋白。该基因的过表达被认为与某些癌症的发生发展存在特殊关系。然而，丝切蛋白1在人类膀胱癌中的具体作用尚未得到深入研究。
在本研究中，我们主要探讨人类膀胱癌与丝切蛋白1表达之间的关系。采用定量聚合酶链反应、蛋白质免疫印迹法和免疫组织化学染色法评估患者膀胱癌组织和癌旁组织中丝切蛋白1的表达。利用小干扰RNA在人RT4膀胱癌细胞系中建立丝切蛋白1表达下调模型，并分析细胞活力变化以确定丝切蛋白1在人类膀胱癌中的作用。
我们的结果显示，3例患者的人类膀胱癌组织中丝切蛋白1在RNA水平和蛋白质水平上的表达均显著高于癌旁组织。丝切蛋白1表达下调可抑制RT4细胞的增殖、迁移、黏附及集落形成能力，并增加细胞凋亡率。
我们的研究表明，丝切蛋白1在人类膀胱癌的发生发展中具有重要作用，该基因可能成为人类膀胱癌诊断和治疗的新靶点。
