Pandiyan Vimal Prabhu, Khare Kedar, John Renu
Indian Institute of Technology Hyderabad, Department of Biomedical Engineering, Kandi, Sangareddy, Telangana 502285, India.
Indian Institute of Technology Delhi, Department of Physics, Hauz Khas, New Delhi 110 016, India.
J Biomed Opt. 2016 Oct 1;21(10):106003. doi: 10.1117/1.JBO.21.10.106003.
We demonstrate a single-shot near on-axis digital holographic microscope that uses a constrained optimization approach for retrieval of the complex object function in the hologram plane. The recovered complex object is back-propagated from the hologram plane to image plane using the Fresnel back-propagation algorithm. A numerical aberration compensation algorithm is employed for correcting the aberrations in the object beam. The reference beam angle is calculated automatically using the modulation property of Fourier transform without any additional recording. We demonstrate this approach using a United States Air Force (USAF) resolution target as an object on our digital holographic microscope. We also demonstrate this approach by recovering the quantitative phase images of live yeast cells, red blood cells and dynamics of live dividing yeast cells.
我们展示了一种单次近轴数字全息显微镜，它使用约束优化方法来恢复全息图平面中的复物体函数。利用菲涅耳逆传播算法将恢复的复物体从全息图平面逆传播到图像平面。采用数值像差补偿算法校正物光束中的像差。利用傅里叶变换的调制特性自动计算参考光束角度，无需额外记录。我们在数字全息显微镜上以美国空军（USAF）分辨率靶标作为物体来演示这种方法。我们还通过恢复活酵母细胞、红细胞的定量相位图像以及活分裂酵母细胞的动力学来演示这种方法。
