Min Junwei, Yao Baoli, Ketelhut Steffi, Engwer Christian, Greve Burkhard, Kemper Björn
Opt Lett. 2017 Jan 15;42(2):227-230. doi: 10.1364/OL.42.000227.
We present a simple and fast phase aberration compensation method in digital holographic microscopy (DHM) for quantitative phase imaging of living cells. By analyzing the frequency spectrum of an off-axis hologram, phase aberrations can be compensated for automatically without fitting or pre-knowledge of the setup and/or the object. Simple and effective computation makes the method suitable for quantitative online monitoring with highly variable DHM systems. Results from automated quantitative phase imaging of living NIH-3T3 mouse fibroblasts demonstrate the effectiveness and the feasibility of the method.
我们提出了一种用于活细胞定量相位成像的数字全息显微镜（DHM）中简单快速的相位像差补偿方法。通过分析离轴全息图的频谱，可以自动补偿相位像差，而无需对设置和/或物体进行拟合或预先了解。简单有效的计算使该方法适用于使用高度可变的DHM系统进行定量在线监测。对活的NIH-3T3小鼠成纤维细胞进行自动定量相位成像的结果证明了该方法的有效性和可行性。
