Yoo Inkyu, Han Jisoo, Lee Soohyung, Jung Wonchul, Kim Ji Hoon, Kim Yeun Wook, Kim Hyun Jong, Hong Minsun, Ka Hakhyun
Division of Biological Science and Technology, Yonsei University, Wonju, 26493, Republic of Korea.
OhsungBio, Chungnam Techno Park, Yesan, 32415, Republic of Korea.
Theriogenology. 2019 Feb;125:173-183. doi: 10.1016/j.theriogenology.2018.11.003. Epub 2018 Nov 11.
Toll-like receptors (TLRs) play critical roles in innate immunity by regulating antimicrobial responses in mucosal tissues. The expression and function of TLRs in female reproductive tissues have been studied in several species, but the expression and function of TLRs and MYD88, an adaptor molecule in the TLR signaling pathway, at the maternal-conceptus interface are not well understood in pigs. Thus, we determined the expression of TLR1 - TLR10 and MYD88 in the endometrium, conceptus, and chorioallantoic tissues of pigs. TLR1 - TLR10 and MYD88 mRNAs were expressed in the endometrium during the estrous cycle and pregnancy in a stage-dependent manner. TLR and MYD88 mRNAs were also detected in early stage conceptuses and chorioallantoic tissues from Day 30 to term pregnancy. The expression of TLR2, TLR4, TLR5, and TLR7 was localized to epithelial and stromal cells in endometrial and chorioallantoic tissues. Increasing doses of P, but not E, induced the expression of TLR4, TLR5, TLR6, TLR7, and TLR8, while interferon-γ increased the expression of TLR2 and TLR7 in endometrial explant tissues. Expression of TLR3, TLR5, TLR6, TLR7, and MYD88 was higher in the endometrium with somatic cell nucleus transfer-derived conceptuses than conceptuses derived from natural mating on Day 12. These results indicate that the expression of TLR1 - TLR10 and MYD88 is dynamically regulated at the maternal-conceptus interface in pigs, suggesting that TLRs expressed in the endometrium and the placenta may play a critical role in regulating mucosal immune responses to support the establishment and maintenance of pregnancy.
Toll样受体（TLRs）通过调节黏膜组织中的抗菌反应在先天免疫中发挥关键作用。TLRs在几种物种的雌性生殖组织中的表达和功能已得到研究，但在猪的母胎界面处，TLRs及TLR信号通路中的衔接分子MYD88的表达和功能尚未得到充分了解。因此，我们测定了猪子宫内膜、孕体和绒毛膜尿囊组织中TLR1 - TLR10和MYD88的表达。TLR1 - TLR10和MYD88的mRNA在发情周期和妊娠期间的子宫内膜中呈阶段依赖性表达。在妊娠第30天至足月的早期孕体和绒毛膜尿囊组织中也检测到了TLR和MYD88的mRNA。TLR2、TLR4、TLR5和TLR7的表达定位于子宫内膜和绒毛膜尿囊组织的上皮细胞和基质细胞。递增剂量的孕酮（P）而非雌激素（E）诱导了TLR4、TLR5、TLR6、TLR7和TLR8的表达，而干扰素-γ增加了子宫内膜外植体组织中TLR2和TLR7的表达。在第12天，与自然交配来源的孕体相比，体细胞核移植来源的孕体的子宫内膜中TLR3、TLR5、TLR6、TLR7和MYD88的表达更高。这些结果表明，猪母胎界面处TLR1 - TLR10和MYD88的表达受到动态调节，提示子宫内膜和胎盘中表达的TLRs可能在调节黏膜免疫反应以支持妊娠的建立和维持中发挥关键作用。
