使用聚乳酸（PLA）热塑性塑料进行三维打印为低温生物学带来了新机遇。
Three-dimensional printing with polylactic acid (PLA) thermoplastic offers new opportunities for cryobiology.
作者信息
Tiersch Terrence R, Monroe William T
机构信息
Aquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, LA 70820, USA.
Department of Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
出版信息
Cryobiology. 2016 Dec;73(3):396-398. doi: 10.1016/j.cryobiol.2016.10.005. Epub 2016 Oct 18.
Abstract
Development of devices through design, prototyping, testing, and fabrication is especially necessary for enhancement of research and eventual application in cryobiology. The advent of 3-dimensional printing offers unique opportunities for this process, given that the materials involved are suitable for use in cryogenic temperatures. We report herein that 3-D printing with polylactic acid (PLA) thermoplastic is ideally suited for cryobiology device development. Devices that are designed and standardized in open-source fashion can be electronically distributed and created locally on increasingly affordable 3-D printers, and can accelerate cryobiology findings and improve reproducibility of results.
摘要
通过设计、原型制作、测试和制造来开发设备对于加强低温生物学的研究及最终应用尤为必要。鉴于所涉及的材料适用于低温温度，三维打印的出现为这一过程提供了独特的机会。我们在此报告，使用聚乳酸（PLA）热塑性塑料进行三维打印非常适合低温生物学设备的开发。以开源方式设计和标准化的设备可以通过电子方式分发，并在日益经济实惠的三维打印机上本地制作，这可以加速低温生物学研究成果，并提高结果的可重复性。
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本文引用的文献
1
Innovations With 3-Dimensional Printing in Physical Medicine and Rehabilitation: A Review of the Literature.物理医学与康复领域三维打印技术的创新：文献综述
PM R. 2016 Dec;8(12):1201-1212. doi: 10.1016/j.pmrj.2016.07.003. Epub 2016 Jul 15.
2
Microfluidics and numerical simulation as methods for standardization of zebrafish sperm cell activation.微流控技术与数值模拟作为斑马鱼精子细胞激活标准化的方法
Biomed Microdevices. 2015;17(3):65. doi: 10.1007/s10544-015-9957-6.
3
Policy: NIH plans to enhance reproducibility.政策：NIH 计划提高可重复性。
Nature. 2014 Jan 30;505(7485):612-3. doi: 10.1038/505612a.
4
Production of F₁ offspring with vitrified sperm from a live-bearing fish, the green swordtail Xiphophorus hellerii.用玻璃化冷冻精子生产 F₁ 代幼鱼，亲鱼为胎生的剑尾鱼（Xiphophorus hellerii）。
Zebrafish. 2011 Dec;8(4):167-79. doi: 10.1089/zeb.2011.0704. Epub 2011 Sep 1.
5
Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method.用于人类卵母细胞和胚胎冷冻保存的高效玻璃化法：Cryotop法
Theriogenology. 2007 Jan 1;67(1):73-80. doi: 10.1016/j.theriogenology.2006.09.014. Epub 2006 Oct 20.
6
Open Pulled Straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos.开放式拉管（OPS）玻璃化冷冻：一种减少牛卵子和胚胎冷冻损伤的新方法。
Mol Reprod Dev. 1998 Sep;51(1):53-8. doi: 10.1002/(SICI)1098-2795(199809)51:1<53::AID-MRD6>3.0.CO;2-V.
7
Engineering-Based Contributions in Cryobiology.
Cryobiology. 1997 Jun;34(4):304-14. doi: 10.1006/cryo.1997.2012.
Zotero 插件