Zargar Tahereh, Khayamian Taghi, Jafari Mohammad T
Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
J Pharm Biomed Anal. 2017 Jan 5;132:232-237. doi: 10.1016/j.jpba.2016.10.014. Epub 2016 Oct 17.
A selective thin-film microextraction based on aptamer immobilized on cellulose paper was used as a paper spray ionization source for ion mobility spectrometry (PSI-IMS), for the first time. In this method, the paper is not only used as an ionization source but also it is utilized for the selective extraction of analyte, based on immobilized aptamer. This combination integrates both sample preparation and analyte ionization in a Whatman paper. To that end, an appropriate sample introduction system with a novel design was constructed for the paper spray ionization source. Using this system, a continuous solvent flow works as an elution and spray solvent simultaneously. In this method, analyte is adsorbed on a triangular paper with immobilized aptamer and then it is desorbed and ionized by elution solvent and applied high voltage on paper, respectively. The effects of different experimental parameters such as applied voltage, angle of paper tip, distance between paper tip and counter electrode, elution solvent type, and solvent flow rate were optimized. The proposed method was exhaustively validated in terms of sensitivity and reproducibility by analyzing the standard solutions of codeine and acetamiprid. The analytical results obtained are promising enough to ensure the use of immobilized aptamer paper-spray as both the extraction and ionization techniques in IMS for direct analysis of biomedicine.
首次将基于固定在纤维素纸上的适配体的选择性薄膜微萃取用作离子迁移谱（PSI-IMS）的纸喷雾电离源。在该方法中，纸不仅用作电离源，还基于固定的适配体用于选择性萃取分析物。这种结合在一张沃特曼纸上集成了样品制备和分析物电离。为此，为纸喷雾电离源构建了一种设计新颖的合适样品引入系统。使用该系统，连续的溶剂流同时用作洗脱溶剂和喷雾溶剂。在该方法中，分析物吸附在带有固定适配体的三角形纸上，然后分别通过洗脱溶剂解吸并通过在纸上施加高压进行电离。对施加电压、纸尖端角度、纸尖端与对电极之间的距离、洗脱溶剂类型和溶剂流速等不同实验参数的影响进行了优化。通过分析可待因和啶虫脒的标准溶液，在灵敏度和重现性方面对所提出的方法进行了全面验证。所获得的分析结果很有前景，足以确保使用固定适配体纸喷雾作为离子迁移谱中用于生物医学直接分析的萃取和电离技术。
