菲律宾一个城市社区无症状个体中芽囊原虫分离株的亚型分析。
Subtype analysis of Blastocystis sp. isolates from asymptomatic individuals in an urban community in the Philippines.
作者信息
Adao Davin Edric V., Dela Serna Ace O., Belleza Maria Luz B., Bolo Nicole R., Rivera Windell L.
机构信息
Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City 1101, Philippines
Molecular Protozoology Laboratory, Natural Sciences Research Institute, University of the Philippines, Diliman, Quezon City 1101, Philippines
出版信息
Ann Parasitol. 2016 Oct 1;62(3):193–200. doi: 10.17420/ap6203.53.
Blastocystis sp. is a commonly reported enteric protistan parasite in faecal specimens with a worldwide distribution afflicting both humans and a wide range of animals. The aim of this study is to characterize the subtypes (STs) of Blastocystis sp. isolates from asymptomatic individuals in an urban community in Pateros, Metro Manila, Philippines. The 600-bp small subunit ribosomal RNA (SSU rRNA) barcoding region of Blastocystis sp. isolates was amplified and sequenced using the primers RD5 and BhRDr. Subtypes were identified by uploading the sequences onto the Basic Local Alignment and Search Tool (BLAST) websites, the Blastocystis Subtype (18S) and Sequence Typing (MLST) Database and by construction of a phylogenetic tree. Twenty-nine (29) out of 35 individuals were detected positive for Blastocystis sp. ST3 is the most common among the three STs detected (65.5%), followed by ST1 (31.0%) and ST4 (3.44%). This study showed that DNA barcoding can serve as a helpful tool to investigate the diversity of Blastocystis sp. in the Philippines.
芽囊原虫是粪便标本中常见的肠道原生动物寄生虫，在全球范围内分布，感染人类和多种动物。本研究旨在鉴定来自菲律宾马尼拉大都会帕泰罗斯一个城市社区无症状个体的芽囊原虫分离株的亚型（STs）。使用引物RD5和BhRDr对芽囊原虫分离株的600bp小亚基核糖体RNA（SSU rRNA）条形码区域进行扩增和测序。通过将序列上传到基本局部比对搜索工具（BLAST）网站、芽囊原虫亚型（18S）和序列分型（MLST）数据库以及构建系统发育树来鉴定亚型。35名个体中有29名检测出芽囊原虫呈阳性。在检测到的三种ST中，ST3最为常见（65.5%），其次是ST1（31.0%）和ST4（3.44%）。本研究表明，DNA条形码可作为研究菲律宾芽囊原虫多样性的有用工具。
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