Falco Aura, Ramos Yusibeska, Franco Esther, Guzmán Alegría, Takiff Howard
Laboratorio de Genética Molecular, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela.
Laboratorio B, Dirección de Energía y Ambiente, Instituto de Estudios Avanzados, Caracas, Venezuela.
BMC Infect Dis. 2016 Oct 22;16(1):595. doi: 10.1186/s12879-016-1927-y.
Klebsiella pneumoniae is a bacterial pathogen that has developed resistance to multiple antibiotics and is a major cause of nosocomial infections worldwide. Carbapenemase-producing Klebsiella pneumoniae have been isolated in many hospitals in Venezuela, but they have not been well-studied. The aim of this study was to characterize carbapenem-resistant Klebsiella pneumoniae isolates from the pediatric service of a hospital located in Anzoategui State, in the eastern part of Venezuela.
Nineteen Klebsiella pneumoniae strains isolated in the hospital from April to July 2014 were evaluated phenotypically and molecularly for the presence of carbapenemases blaKPC, blaIMP and blaVIM. Molecular epidemiology was performed with Repetitive Extragenic Palindromic-PCR (REP-PCR) and Multilocus Sequence Typing (MLST). They were also studied for phenotypic and molecular resistance to a quaternary ammonium compound (QAC) disinfectant.
All 19 isolates contained both bla and bla genes, and the bla gene was associated with Tn4401b. All isolates were phenotypically sensitive to QACs and contained qacΔE and addA2 genes typical of class 1 integrons. Analysis by REP-PCR and MLST showed that all isolates had identical profiles characteristic of sequence type ST833.
All 19 strains are bla and bla -producing ST833 K. pneumoniae sensitive to QACs. This analysis may help to understand the routes of dissemination and confirms that QAC disinfectants can be used to help control their spread.
肺炎克雷伯菌是一种对多种抗生素产生耐药性的细菌病原体，是全球医院感染的主要原因。委内瑞拉的许多医院都分离出了产碳青霉烯酶的肺炎克雷伯菌，但对其研究尚不充分。本研究的目的是对委内瑞拉东部安索阿特吉州一家医院儿科服务中分离出的耐碳青霉烯肺炎克雷伯菌菌株进行特征分析。
对2014年4月至7月在该医院分离出的19株肺炎克雷伯菌进行表型和分子分析，检测碳青霉烯酶blaKPC、blaIMP和blaVIM的存在情况。采用重复外显子回文PCR（REP-PCR）和多位点序列分型（MLST）进行分子流行病学研究。还研究了它们对季铵化合物（QAC）消毒剂的表型和分子耐药性。
所有19株分离株均同时含有bla 和bla 基因，且bla 基因与Tn4401b相关。所有分离株对QACs表型敏感，且含有1类整合子典型的qacΔE和addA2基因。REP-PCR和MLST分析表明，所有分离株具有相同的序列类型ST833特征图谱。
所有19株菌株均为产bla 和bla 的ST833型肺炎克雷伯菌，对QACs敏感。该分析可能有助于了解传播途径，并证实QAC消毒剂可用于帮助控制其传播。
