Komoto Satoshi, Pongsuwanna Yaowapa, Tacharoenmuang Ratana, Guntapong Ratigorn, Ide Tomihiko, Higo-Moriguchi Kyoko, Tsuji Takao, Yoshikawa Tetsushi, Taniguchi Koki
Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
Virus Research Institute, National Institute of Health, Department of Medical Sciences, Nonthaburi 11000, Thailand.
Vet Microbiol. 2016 Nov 15;195:37-57. doi: 10.1016/j.vetmic.2016.09.003. Epub 2016 Sep 13.
Bovine group A rotavirus (RVA) is an important cause of acute diarrhea in calves worldwide. In order to obtain precise information on the origin and evolutionary dynamics of bovine RVA strains, we determined and analyzed the complete nucleotide sequences of the whole genomes of six archival bovine RVA strains; four Thai strains (RVA/Cow-tc/THA/A5-10/1988/G8P[1], RVA/Cow-tc/THA/A5-13/1988/G8P[1], RVA/Cow-tc/THA/61A/1989/G10P[5], and RVA/Cow-tc/THA/A44/1989/G10P[11]), one American strain (RVA/Cow-tc/USA/B223/1983/G10P[11]), and one Japanese strain (RVA/Cow-tc/JPN/KK3/1983/G10P[11]). On whole genomic analysis, the 11 gene segments of strains A5-10, A5-13, 61A, A44, B223, and KK3 were found to be considerably genetically diverse, but to share a conserved non-G/P genotype constellation except for the NSP1 gene (I2-R2-C2-M2-(A3/11/13/14)-N2-T6-E2-H3), which is commonly found in RVA strains from artiodactyls such as cattle. Furthermore, phylogenetic analysis revealed that most genes of the six strains were genetically related to bovine and bovine-like strains. Of note is that the VP1, VP3, and NSP2 genes of strains A5-10 and A5-13 exhibited a closer relationship with the cognate genes of human DS-1-like strains than those of other RVA strains. Furthermore, the VP6 genes of strains A5-10 and A5-13 appeared to be equally related to both human DS-1-like and bovine strains. Thus, strains A5-10 and A5-13 were suggested to be derived from the same evolutionary origin as human DS-1-like strains, and were assumed to be examples of bovine RVA strains that provide direct evidence for a close evolutionary relationship between bovine and human DS-1-like strains. Our findings will provide important insights into the origin of bovine RVA strains, and into evolutionary links between bovine and human RVA strains.
牛A组轮状病毒（RVA）是全球范围内犊牛急性腹泻的重要病因。为了获取有关牛RVA毒株起源和进化动态的确切信息，我们测定并分析了6株存档牛RVA毒株全基因组的完整核苷酸序列；4株泰国毒株（RVA/Cow-tc/THA/A5-10/1988/G8P[1]、RVA/Cow-tc/THA/A5-13/1988/G8P[1]、RVA/Cow-tc/THA/61A/1989/G10P[5]和RVA/Cow-tc/THA/A44/1989/G10P[11]）、1株美国毒株（RVA/Cow-tc/USA/B223/1983/G10P[11]）和1株日本毒株（RVA/Cow-tc/JPN/KK3/1983/G10P[11]）。通过全基因组分析发现，A5-10、A5-13、61A、A44、B223和KK3毒株的11个基因片段在遗传上有很大差异，但除NSP1基因外共享一个保守的非G/P基因型组合（I2-R2-C2-M2-(A3/11/13/14)-N2-T6-E2-H3），该基因在牛等偶蹄目动物的RVA毒株中普遍存在。此外，系统发育分析表明，这6株毒株的大多数基因与牛及牛样毒株在遗传上相关。值得注意的是，A5-10和A5-13毒株的VP1、VP3和NSP2基因与人类DS-1样毒株的同源基因的关系比与其他RVA毒株的同源基因更密切。此外，A5-10和A5-13毒株的VP6基因似乎与人类DS-1样毒株和牛毒株的关系相同。因此，A5-10和A5-13毒株被认为与人类DS-1样毒株起源于同一进化源，并且被假定为牛RVA毒株的实例，为牛与人类DS-1样毒株之间密切的进化关系提供了直接证据。我们的研究结果将为牛RVA毒株的起源以及牛和人类RVA毒株之间的进化联系提供重要见解。
