双色流式核型中人类Y染色体不同的Hoechst 33342和DAPI荧光

Different Hoechst 33342 and DAPI fluorescence of the human Y chromosome in bivariate flow karyotypes.

作者信息

Bernheim A, Miglierina R

机构信息

Laboratoire de Cytogénétique, U3OI INSERM and L.O.I., UM7 CNRS, Paris, France.

出版信息

Hum Genet. 1989 Sep;83(2):189-93. doi: 10.1007/BF00286716.

DOI:10.1007/BF00286716

PMID:2777260

Abstract

The staining properties of AT-specific dyes Hoechst 33342 and DAPI as revealed by Hoechst 33342/mithramycin and mithramycin/DAPI bivariate human flow karyotype patterns are different for chromosomes rich in heterochromatin. The peak corresponding to chromosome Y of a given cell line is higher on the A/T axis with mithramycin/DAPI staining than with mithramycin/Hoechst. The chromosome 1 was found slightly more fluorescent in mithramycin/DAPI than in mithramycin/Hoechst as judged by the slight displacement of its area on the Hoechst-DAPI axis. The other peaks did not show major differences. On the same flow karyotypes, chromosomal rearrangements were detected.

摘要

由Hoechst 33342/光神霉素和光神霉素/DAPI双变量人类流式核型模式所揭示的富含异染色质的染色体上，AT特异性染料Hoechst 33342和DAPI的染色特性有所不同。对于给定细胞系，在光神霉素/DAPI染色中，对应于Y染色体的峰在A/T轴上比光神霉素/Hoechst染色时更高。根据其在Hoechst-DAPI轴上区域的轻微位移判断，在光神霉素/DAPI染色中，1号染色体比光神霉素/Hoechst染色时荧光略强。其他峰未显示出主要差异。在相同的流式核型上，检测到了染色体重排。