Langlois R G, Yu L C, Gray J W, Carrano A V
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7876-80. doi: 10.1073/pnas.79.24.7876.
Dual beam flow cytometry of chromosomes stained with Hoechst 33258 and chromomycin A3 has been proposed as a method for quantitative classification of human chromosomes (bivariate flow karyotyping). In this paper we investigate the sources and magnitudes of variability in the mean fluorescence intensities of each chromosome group resolved in bivariate flow karyotypes and study the impact of this variability on chromosome classification. Replicate bivariate flow karyotypes of chromosomes isolated from lymphocytes from 10 individuals demonstrated that person-to-person variability was significantly greater than run-to-run variability. The total variability was sufficiently small that it did not interfere with classification of normal chromosome types except chromosomes 9 through 12 and chromosomes 14 and 15. Furthermore, the variability was generally smaller than 1/600th of the mitotic genome, so that one-band rearrangements should be detectable in bivariate flow karyotypes.
有人提出，用Hoechst 33258和放线菌素A3染色的染色体进行双光束流式细胞术可作为人类染色体定量分类的一种方法（双变量流式核型分析）。在本文中，我们研究了双变量流式核型中分辨出的每个染色体组平均荧光强度的变异来源和大小，并研究了这种变异对染色体分类的影响。对从10名个体的淋巴细胞中分离出的染色体进行重复双变量流式核型分析，结果表明个体间的变异明显大于批次间的变异。总的变异足够小，除了9号至12号染色体以及14号和15号染色体外，不会干扰正常染色体类型的分类。此外，变异通常小于有丝分裂基因组的1/600，因此在双变量流式核型中应该可以检测到单带重排。
