Joshi P J, Samuel A, Dongre D S, Sutaria S N
Indian J Pathol Microbiol. 1989 Jul;32(3):174-8.
A total of 400 strains of Gram negative bacilli were examined both by conventional disc diffusion (DD) and by rapid fermentation sensitivity test (RFST) methods. Various strains which were examined included Esch. coli Kleb. aerogenes, Kleb. pneumoniae, Prot. vulgaris, Prot. mirabilis, Prot. morgani, Prot. rittgeri and Providencia. The antibiotics used were streptomycin, chloramphenicol, kanamycin, gentamycin and ampicillin. The correlation between the two methods was found to vary from 75 to 98 per cent. By RFST method the results are obtained within six hours. The limitations of this method are (i) it cannot be used for Pseudomonas aeruginosa as it does not ferment glucose, (ii) it cannot be used for antibiotics active only at acidic pH like tetracycline and (iii) partial or intermediate sensitivity cannot be tested by this method. On the other hand, it is a simple, inexpensive, rapid and reliable method feasible in small laboratories also. The results are not affected by the size of inoculum or quality of medium (agar) used. Thus, it is very helpful especially when immediate antibiotic sensitivity is required.
共采用传统纸片扩散法（DD）和快速发酵药敏试验（RFST）方法检测了400株革兰氏阴性杆菌。检测的各类菌株包括大肠杆菌、产气克雷伯菌、肺炎克雷伯菌、普通变形杆菌、奇异变形杆菌、摩根氏变形杆菌、雷氏变形杆菌和普罗威登斯菌属。使用的抗生素有链霉素、氯霉素、卡那霉素、庆大霉素和氨苄青霉素。发现这两种方法之间的相关性在75%至98%之间。通过RFST方法可在6小时内获得结果。该方法的局限性在于：（i）不能用于铜绿假单胞菌，因为它不发酵葡萄糖；（ii）不能用于仅在酸性pH值下有活性的抗生素，如四环素；（iii）该方法无法检测部分或中度敏感性。另一方面，它是一种简单、廉价、快速且可靠的方法，在小型实验室也可行。结果不受接种物大小或所用培养基（琼脂）质量的影响。因此，特别是在需要立即获得抗生素敏感性结果时，它非常有用。
