Saoji A M, Naghi T
Indian J Pathol Microbiol. 1989 Jul;32(3):221-6.
Based on our earlier work we hereby describe pre-staining of normal human serum with Remazol Brilliant Blue (RBB) to precisely locate the third component of complement system (C3,B1C) on polyacrylamide gels. Testing the dye-protein complex eluted from different immunoreactive post-transferrin (origin to transferrin) components against the standards anti-C3 antibody in various gel precipitation techniques, revealed that the prominent band just posterior to the centrally placed transferrin is of complement. Subsequent to its identity, we also evolved a method to raise an anti-C3 antibody. The resulting antibody was found to be monospecific when assessed in different gel precipitation procedures.
基于我们早期的工作，我们在此描述用雷马素亮蓝（RBB）对正常人血清进行预染色，以便在聚丙烯酰胺凝胶上精确定位补体系统的第三成分（C3，B1C）。在各种凝胶沉淀技术中，用从不同免疫反应性转铁蛋白后成分（从原点到转铁蛋白）洗脱的染料 - 蛋白质复合物与标准抗C3抗体进行测试，结果显示，位于中央转铁蛋白后方的明显条带是补体的条带。在确定其身份之后，我们还开发了一种制备抗C3抗体的方法。在不同的凝胶沉淀程序中评估时，发现所得抗体具有单特异性。
