Dong Zhiying, Wang Kai, Chen Xinxin, Zhu Jianlin, Hu Changju, Zhang Demin
School of Marine Sciences, Ningbo University, Ningbo 315211, China; Faculty of Architectural, Civil Engineering and Environment, Ningbo University, Ningbo 315211, China.
School of Marine Sciences, Ningbo University, Ningbo 315211, China; Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo 315211, China.
Mar Pollut Bull. 2017 Jan 30;114(2):656-663. doi: 10.1016/j.marpolbul.2016.10.041. Epub 2016 Oct 20.
Coastal ecosystems are receiving elevated loads of nitrogen (N) from anthropogenic sources. Understanding how excessive N loading affects bacterioplankton communities is critical to predict the biodiversity of marine ecosystems under conditions of eutrophic disturbance. In this study, oligotrophic coastal water microcosms were perturbed with nitrate in two loading modes: 1) one-off loading at the beginning of the incubation period; and 2) periodic loading every two days for 16days. Turnover in the bacterioplankton community was investigated by 16S rDNA gene amplicon sequencing. The alpha diversity of the bacterioplankton community showed great temporal variability and similar responses to the different treatments. Bacterioplankton community composition was influenced remarkably by time and N loading mode. The effects of N loading on bacterioplankton community structure showed obvious temporal variation, probably because of the great temporal variation in environmental parameters. This study provides insights into the effects of N pollution in anthropogenically perturbed marine environments.
沿海生态系统正承受着来自人为源的不断增加的氮负荷。了解过量的氮负荷如何影响浮游细菌群落对于预测富营养化干扰条件下海洋生态系统的生物多样性至关重要。在本研究中，贫营养的沿海水微宇宙以两种负荷模式用硝酸盐进行扰动：1）在培养期开始时一次性加载；2）每两天周期性加载，持续16天。通过16S rDNA基因扩增子测序研究浮游细菌群落的周转率。浮游细菌群落的α多样性表现出很大的时间变异性，并且对不同处理的反应相似。浮游细菌群落组成受到时间和氮负荷模式的显著影响。氮负荷对浮游细菌群落结构的影响表现出明显的时间变化，这可能是由于环境参数的巨大时间变化所致。本研究为了解人为扰动的海洋环境中氮污染的影响提供了见解。
