He Yaodong, Sen Biswarup, Zhou Shuangyan, Xie Ningdong, Zhang Yongfeng, Zhang Jianle, Wang Guangyi
Center for Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin UniversityTianjin, China.
Qinhuangdao Marine Environmental Monitoring Central Station, State Oceanic AdministrationQinhuangdao, China.
Front Microbiol. 2017 Aug 18;8:1579. doi: 10.3389/fmicb.2017.01579. eCollection 2017.
Qinhuangdao coastal waters in northern China are heavily impacted by anthropogenic and natural activities, and we anticipate a direct influence of the impact on the bacterioplankton abundance and diversity inhabiting the adjacent coastal areas. To ascertain the anthropogenic influences, we first evaluated the seasonal abundance patterns and diversity of bacterioplankton in the coastal areas with varied levels of natural and anthropogenic activities and then analyzed the environmental factors which influenced the abundance patterns. Results indicated distinct patterns in bacterioplankton abundance across the warm and cold seasons in all stations. Total bacterial abundance in the stations ranged from 8.67 × 10 to 2.08 × 10 cells/mL and had significant ( < 0.01) positive correlation with total phosphorus (TP), which indicated TP as the key monitoring parameter for anthropogenic impact on nutrients cycling. and were the most abundant phyla in the Qinhuangdao coastal waters. Redundancy analysis revealed significant ( < 0.01) influence of temperature, dissolved oxygen and chlorophyll on the spatiotemporal abundance pattern of α Among the 19 identified bacterioplankton subgroups, α (phylum ) was the dominant one followed by (phylum ), representing 19.1-55.2% and 2.3-54.2% of total sequences, respectively. An inverse relationship ( = -0.82) was observed between the two dominant subgroups, α. A wide range of inverse Simpson index (10.2 to 105) revealed spatial heterogeneity of bacterioplankton diversity likely resulting from the varied anthropogenic and natural influences. Overall, our results suggested that seasonal variations impose substantial influence on shaping bacterioplankton abundance patterns. In addition, the predominance of only a few cosmopolitan species in the Qinhuangdao coastal wasters was probably an indication of their competitive advantage over other bacterioplankton groups in the degradation of anthropogenic inputs. The results provided an evidence of their ecological significance in coastal waters impacted by seasonal inputs of the natural and anthropogenic matter. In conclusion, the findings anticipate future development of effective indicators of coastal health monitoring and subsequent management strategies to control the anthropogenic inputs in the Qinhuangdao coastal waters.
中国北方的秦皇岛沿海水域受到人为活动和自然活动的严重影响，我们预计这种影响会对栖息在邻近沿海地区的浮游细菌的丰度和多样性产生直接影响。为了确定人为影响，我们首先评估了自然和人为活动水平不同的沿海地区浮游细菌的季节性丰度模式和多样性，然后分析了影响丰度模式的环境因素。结果表明，所有站点的浮游细菌丰度在温暖和寒冷季节呈现出明显的模式。各站点的总细菌丰度范围为8.67×10至2.08×10个细胞/毫升，与总磷（TP）呈显著（<0.01）正相关，这表明TP是人为影响营养物质循环的关键监测参数。 和 是秦皇岛沿海水域中最丰富的门类。冗余分析表明，温度、溶解氧和叶绿素 对α的时空丰度模式有显著（<0.01）影响。在19个已鉴定的浮游细菌亚组中，α（ 门）是优势亚组，其次是 （ 门），分别占总序列的19.1 - 55.2%和2.3 - 54.2%。在两个优势亚组α之间观察到负相关关系（ = -0.82）。广泛的反辛普森指数（10.2至105）揭示了浮游细菌多样性的空间异质性，这可能是由不同的人为和自然影响造成的。总体而言，我们的结果表明季节性变化对塑造浮游细菌丰度模式有重大影响。此外，秦皇岛沿海废水中只有少数世界性物种占优势，这可能表明它们在降解人为输入物方面比其他浮游细菌群体具有竞争优势。这些结果证明了它们在受自然和人为物质季节性输入影响的沿海水域中的生态意义。总之，这些发现为未来开发有效的沿海健康监测指标以及随后控制秦皇岛沿海水域人为输入的管理策略提供了依据。
