Matsuoka H, Sugimachi K, Ueo H, Mori M, Akiyoshi T
Department of Surgery, Kyushu University, Beppu, Japan.
Int J Hyperthermia. 1990 Nov-Dec;6(6):1019-29. doi: 10.3109/02656739009140985.
We have developed a hyperthermic sensitivity test for human neoplastic cells using contact-sensitive confluent monolayers of BALB/c 3T3 cells designated as 'contact-sensitive plates (CSP)', which almost completely inhibited the growth of normal cells without influencing the growth of the human neoplastic cells. On 'CSP', the relative [3H]uridine incorporation into RNA of the hyperthermic cultures showed an excellent correlation with the relative clonogenic efficiency of the treated cultures. Since 'CSP' allows for only contact-insensitive tumour cells to proliferate, our assay will predict the hyperthermic sensitivity of only the neoplastic cells. Moreover, even 2 x 10(3) cells derived from clinical tumour tissues can be assayed within only 3 days. This assay system is potentially used as a rapid in vitro hyperthermic sensitivity test for human carcinoma.
我们利用指定为“接触敏感平板（CSP）”的BALB/c 3T3细胞的接触敏感汇合单层，开发了一种用于人类肿瘤细胞的热敏感性测试方法，该方法几乎完全抑制正常细胞的生长，而不影响人类肿瘤细胞的生长。在“CSP”上，热培养物中相对[3H]尿苷掺入RNA的情况与处理后培养物的相对克隆形成效率显示出极好的相关性。由于“CSP”仅允许接触不敏感的肿瘤细胞增殖，我们的检测方法将仅预测肿瘤细胞的热敏感性。此外，即使是从临床肿瘤组织中获取的2×10³个细胞，也只需3天就能完成检测。该检测系统有可能用作人类癌症的快速体外热敏感性测试。
