Kolondam Beivy, Rao Parth, Sztuba-Solinska Joanna, Weber Philipp H, Dzianott Aleksandra, Johns Mitrick A, Bujarski Jozef J
Department of Biological Sciences and Plant Molecular Biology Center, Northern Illinois University, DeKalb, IL 60115, USA and.
Department of Biological Sciences and Plant Molecular Biology Center, Northern Illinois University, DeKalb, IL 60115, USA and; Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
Virus Evol. 2015 Dec 23;1(1):vev021. doi: 10.1093/ve/vev021. eCollection 2015.
We have previously reported intra-segmental crossovers in Brome mosaic virus (BMV) RNAs. In this work, we studied the homologous recombination of BMV RNA in three different hosts: barley (, , and that were co-infected with two strains of BMV: Russian (R) and Fescue (F). Our work aimed at (1) establishing the frequency of recombination, (2) mapping the recombination hot spots, and (3) addressing host effects. The F and R nucleotide sequences differ from each other at many translationally silent nucleotide substitutions. We exploited this natural variability to track the crossover sites. Sequencing of a large number of cDNA clones revealed multiple homologous crossovers in each BMV RNA segment, in both the whole plants and protoplasts. Some recombination hot spots mapped at similar locations in different hosts, suggesting a role for viral factors, but other sites depended on the host. Our results demonstrate the chimeric ('mosaic') nature of the BMV RNA genome.
我们之前报道过雀麦花叶病毒（BMV）RNA的片段内交叉现象。在这项工作中，我们研究了BMV RNA在三种不同宿主中的同源重组：大麦（品种为、和），这些大麦被两种BMV毒株共同感染，即俄罗斯毒株（R）和羊茅毒株（F）。我们的工作旨在：（1）确定重组频率；（2）绘制重组热点图谱；（3）研究宿主效应。F和R核苷酸序列在许多翻译沉默的核苷酸替换上彼此不同。我们利用这种自然变异性来追踪交叉位点。对大量cDNA克隆进行测序后发现，在整株植物和原生质体的每个BMV RNA片段中都存在多个同源交叉。一些重组热点在不同宿主中的定位相似，这表明病毒因子发挥了作用，但其他位点则取决于宿主。我们的结果证明了BMV RNA基因组的嵌合（“镶嵌”）性质。
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