Dehghan Mohammad Hossein, Mirmiranpour Hossein, Faghihi-Kashani Sara, Kabir Kourosh, Larry Mehrdad, Zayerzadeh Ehsan, Salehi Salume
Biochemistry Department, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Biochemistry Department, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran; Endocrinology and Metabolism Research Center (EMRC), Vali-Asr Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
J Tradit Complement Med. 2016 Jan 27;6(4):437-441. doi: 10.1016/j.jtcme.2015.12.003. eCollection 2016 Oct.
Curcumin (diferuloylmethane) has been associated with the inhibition of angiogenesis, as well as the prevention of cancers and inflammatory processes. The aim of this study was to assess the efficacy of curcumin in suppressing angiogenesis in the cultured endothelial cells of rat aortic rings.
Eight-week-old male Wistar rats were randomized into five groups each with a different treatment and cell culturing paradigm: controls cultured in the absence of VEGF (vascular endothelial growth factor) (C), controls cultured in the presence of VEGF (C-V), controls treated with curcumin and then cultured in media lacking VEGF (C-TC), diabetics cultured in media supplemented with VEGF (D-V) and diabetics treated with curcumin and then cultured in media supplemented with VEGF (D-V-TC). Each group consisted of 8 animals. Diabetes was induced in by streptozotocin (STZ; 60 mg/kg body weight, IV). After 8 weeks, animals were sacrificed and their aortas were excised. Ring-shaped explants were embedded in a 96-well culture plate. Angiogenesis response was measured by counting the number of primary microtubules in each well.
Optic microscopy revealed that the D-V group had the highest number of microvessels, while angiogenesis was not observed in the C or C-TC groups. The number of primary microtubules was significantly lower in the D-V-TC group compared to the D-V group (P < 0.05). The D-V-TC group had a significantly higher number of microvessels compared to the C-TC group (P < 0.05).
Curcumin attenuates angiogenesis response in stertozotocin-induced diabetic rats.
姜黄素（二阿魏酰甲烷）与血管生成抑制以及癌症和炎症过程的预防有关。本研究的目的是评估姜黄素在抑制大鼠主动脉环培养内皮细胞血管生成中的功效。
将8周龄雄性Wistar大鼠随机分为五组，每组采用不同的处理和细胞培养模式：在无血管内皮生长因子（VEGF）的情况下培养的对照组（C）、在有VEGF的情况下培养的对照组（C-V）、用姜黄素处理后在缺乏VEGF的培养基中培养的对照组（C-TC）、在补充有VEGF的培养基中培养的糖尿病组（D-V）以及用姜黄素处理后在补充有VEGF的培养基中培养的糖尿病组（D-V-TC）。每组由8只动物组成。通过链脲佐菌素（STZ；60mg/kg体重，静脉注射）诱导糖尿病。8周后，处死动物并切除其主动脉。将环形外植体嵌入96孔培养板中。通过计数每个孔中的初级微管数量来测量血管生成反应。
光学显微镜显示，D-V组的微血管数量最多，而在C组或C-TC组中未观察到血管生成。与D-V组相比，D-V-TC组的初级微管数量显著降低（P<0.05）。与C-TC组相比，D-V-TC组的微血管数量显著更高（P<0.05）。
姜黄素可减弱链脲佐菌素诱导的糖尿病大鼠的血管生成反应。
