Chaudhary Amrita, Bag Swarnendu, Banerjee Provas, Chatterjee Jyotirmoy
School of Medical Science and Technology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, West Bengal, India.
Histopathology Lab, Hospital Phase 2, Tata Medical Center, New Town, Kolkata, West Bengal, 700160, India.
J Tradit Complement Med. 2019 Oct 10;10(6):529-543. doi: 10.1016/j.jtcme.2019.10.002. eCollection 2020 Nov.
Diabetic patients are frequently afflicted with impaired wound healing where linear progression of molecular and cellular events compromised. Despite of meaningful progress in diabetic treatment, management of diabetic chronic wounds is still challenging. Jamun () honey may be a promising candidate for diabetic wound healing and need to explore in detail. So present study was designed to evaluate the efficacy of Jamun honey (JH) for diabetic wound healing in wound (primary fibroblasts) model and in of diabetic mice (Streptozotocin induced) model. The fibroblast cell model was studied for migratory behaviour and myofibrolasts infiltration under honey interventions scratch/migration assay, immuno-cytochemistry and western blot. We applied FDA approved Manuka honey (MH) as positive control and JH as test honey to evaluate wound re-epithelialization, sub-epithelial connective tissue modification and angiogenesis histo-pathological and immuno-histochemical analysis. JH (0.1% v/v) dilution has notably improved wound closure, migration with concomitant α-SMA expressions . Topical application of JH in diabetic mice model showed significant (*p ≤ 0.05) wound closure, reepithelialization, collagen deposition (I/III) and balanced the myofibroblasts formation. It also modulated vital angiogenic markers ( HIF-1α, VEGF, VEGF R-II) significantly (*p ≤ 0.05). All these observations depicted that JH promotes sequential stages of wound healing in diabetic mice model. The results of the present study established Jamun honey as good as Manuka honey considering wound closure, re-epithelialization, collagen deposition and pro-angiogenic potential.
糖尿病患者经常受到伤口愈合受损的困扰，分子和细胞事件的线性进展受到损害。尽管糖尿病治疗取得了有意义的进展，但糖尿病慢性伤口的管理仍然具有挑战性。印度醋栗（Jamun）蜂蜜可能是糖尿病伤口愈合的一个有前途的候选者，需要进行详细研究。因此，本研究旨在评估印度醋栗蜂蜜（JH）在伤口（原代成纤维细胞）模型和糖尿病小鼠（链脲佐菌素诱导）模型中的糖尿病伤口愈合效果。在蜂蜜干预下，通过划痕/迁移试验、免疫细胞化学和蛋白质印迹法研究成纤维细胞模型的迁移行为和肌成纤维细胞浸润情况。我们应用美国食品药品监督管理局（FDA）批准的麦卢卡蜂蜜（MH）作为阳性对照，JH作为试验蜂蜜，通过组织病理学和免疫组织化学分析来评估伤口再上皮化、上皮下结缔组织改变和血管生成情况。JH（0.1% v/v）稀释液显著改善了伤口闭合、迁移，并伴有α-SMA表达。在糖尿病小鼠模型中局部应用JH显示出显著（*p≤0.05）的伤口闭合、再上皮化、胶原蛋白沉积（I/III），并平衡了肌成纤维细胞的形成。它还显著调节了重要的血管生成标志物（HIF-1α、VEGF、VEGF R-II）（*p≤0.05）。所有这些观察结果表明，JH促进了糖尿病小鼠模型伤口愈合的各个阶段。本研究结果表明，在伤口闭合、再上皮化、胶原蛋白沉积和促血管生成潜力方面，印度醋栗蜂蜜与麦卢卡蜂蜜一样好。
