缺氧研究中的常见问题。

Wenger Roland H, Kurtcuoglu Vartan, Scholz Carsten C, Marti Hugo H, Hoogewijs David

Institute of Physiology and Zurich Center for Human Physiology (ZIHP), University of Zurich; National Center of Competence in Research "Kidney. CH", Zurich, Switzerland.

Institute of Physiology and Pathophysiology, University of Heidelberg, Heidelberg.

Hypoxia (Auckl). 2015 Sep 18;3:35-43. doi: 10.2147/HP.S92198. eCollection 2015.

"What is the O concentration in a normoxic cell culture incubator?" This and other frequently asked questions in hypoxia research will be answered in this review. Our intention is to give a simple introduction to the physics of gases that would be helpful for newcomers to the field of hypoxia research. We will provide background knowledge about questions often asked, but without straightforward answers. What is O concentration, and what is O partial pressure? What is normoxia, and what is hypoxia? How much O is experienced by a cell residing in a culture dish in vitro vs in a tissue in vivo? By the way, the O concentration in a normoxic incubator is 18.6%, rather than 20.9% or 20%, as commonly stated in research publications. And this is strictly only valid for incubators at sea level.

“常氧细胞培养箱中的氧气浓度是多少？”以及低氧研究中的其他常见问题将在本综述中得到解答。我们的目的是对气体物理学进行简单介绍，这将有助于低氧研究领域的新手。我们将提供有关常见问题的背景知识，但没有直接答案。什么是氧气浓度，什么是氧分压？什么是常氧，什么是低氧？与体内组织中的细胞相比，体外培养皿中的细胞经历了多少氧气？顺便说一下，常氧培养箱中的氧气浓度是18.6%，而不是研究出版物中通常所说的20.9%或20%。而且这严格来说仅适用于海平面的培养箱。