Centre for Biomedical Engineering, Department of Mechanical Engineering Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK.
Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK.
Analyst. 2016 Nov 14;141(23):6408-6415. doi: 10.1039/c6an01596d.
A loss of ability of cells to undergo apoptosis (programmed cell death, whereby the cell ceases to function and destroys itself) is commonly associated with cancer, and many anti-cancer interventions aim to restart the process. Consequently, the accurate quantification of apoptosis is essential in understanding the function and performance of new anti-cancer drugs. Dielectrophoresis has previously been demonstrated to detect apoptosis more rapidly than other methods, and is low-cost, label-free and rapid, but has previously been unable to accurately quantify cells through the apoptotic process because cells in late apoptosis disintegrate, making cell tracking impossible. In this paper we use a novel method based on light absorbance and multi-population tracking to quantify the progress of apoptosis, benchmarking against conventional assays including MTT, trypan blue and Annexin-V. Analyses are performed on suspension and adherent cells, and using two apoptosis-inducing agents. IC measurements compared favourably to MTT and were superior to trypan blue, whilst also detecting apoptotic progression faster than Annexin-V.
细胞丧失凋亡能力（程序性细胞死亡，即细胞停止功能并自我毁灭）通常与癌症有关，许多抗癌干预措施旨在重新启动该过程。因此，准确量化细胞凋亡对于理解新型抗癌药物的功能和性能至关重要。电动力学以前已被证明比其他方法更快地检测到细胞凋亡，而且成本低、无需标记且快速，但以前由于晚期凋亡的细胞会解体，使细胞跟踪变得不可能，因此无法准确量化细胞凋亡过程。在本文中，我们使用了一种基于吸光度和多群体跟踪的新方法来定量细胞凋亡的进程，该方法与包括 MTT、台盼蓝和 Annexin-V 在内的传统方法进行了基准测试。对悬浮和贴壁细胞进行了分析，并使用了两种诱导凋亡的药物。IC 测量结果与 MTT 相比具有优势，优于台盼蓝，同时也比 Annexin-V 更快地检测到细胞凋亡的进展。
