Future Industries Institute, University of South Australia, Mawson Lakes, SA, Australia.
Methods Mol Biol. 2023;2644:15-33. doi: 10.1007/978-1-0716-3052-5_2.
The MTT assay is extensively used, most often to infer a measure of cytotoxicity of treatments to cells. As with any assay though, there are a number of limitations. The method described here is designed with consideration of how the MTT assay fundamentally works to account for, or at least identify, confounding factors in measurements. It also provides a decision-making framework to best interpret and complement the MTT assay to apply it as either a measure of metabolic activity or cell viability.
MTT 测定法被广泛应用，通常用于推断细胞处理的细胞毒性程度。然而，与任何测定法一样，它存在许多局限性。此处描述的方法是根据 MTT 测定法的基本工作原理设计的，旨在考虑或至少识别测量中的混杂因素。它还提供了一个决策框架，以最佳地解释和补充 MTT 测定法，将其用作代谢活性或细胞活力的衡量标准。
