Labeed Fatima H, Coley Helen M, Hughes Michael P
Centre for Biomedical Engineering, School of Engineering, University of Surrey, Guildford, Surrey GU2 7XH, UK.
Biochim Biophys Acta. 2006 Jun;1760(6):922-9. doi: 10.1016/j.bbagen.2006.01.018. Epub 2006 Feb 23.
We have used dielectrophoresis to determine the dielectric properties of human chronic myelogeneous leukaemic (K562) cells during apoptosis (programmed cell death). Our results indicate that K562 cells increase markedly in cytoplasmic conductivity from 0.28 S/m to 0.50 S/m within the first 4 h following treatment with staurosporine, which then lasts beyond 12 h, whilst cell shrinkage increases the capacitance of the membrane from 9.7 mF/m2 to 20 mF/m2. After 24 and 48 h of incubation with staurosporine, multiple sub-populations were detected, highlighted by the dielectric changes that the cell undergoes before death. By comparing these results with those obtained by common apoptosis monitoring techniques Annexin V and TMRE (tetramethylrhodamine ethylester), it is possible to infer the role of ion efflux in the progress of apoptosis. The use of dielectrophoresis for monitoring apoptosis offers a number of benefits as it is both rapid and non-invasive. It can also be used in parallel with other assays in high-throughput screening applications.
我们利用介电电泳来测定人慢性髓性白血病（K562）细胞在凋亡（程序性细胞死亡）过程中的介电特性。我们的结果表明，在用星形孢菌素处理后的最初4小时内，K562细胞的细胞质电导率从0.28 S/m显著增加到0.50 S/m，随后持续超过12小时，而细胞收缩使细胞膜电容从9.7 mF/m²增加到20 mF/m²。在用星形孢菌素孵育24小时和48小时后，检测到多个亚群，细胞死亡前经历的介电变化突出了这些亚群。通过将这些结果与通过常用的凋亡监测技术膜联蛋白V和四甲基罗丹明乙酯（TMRE）获得的结果进行比较，可以推断离子外流在凋亡进程中的作用。使用介电电泳监测凋亡具有许多优点，因为它既快速又非侵入性。它还可以与其他检测方法并行用于高通量筛选应用中。
