Hervé Vincent, Duruflé Harold, San Clemente Hélène, Albenne Cécile, Balliau Thierry, Zivy Michel, Dunand Christophe, Jamet Elisabeth
Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Castanet Tolosan, France.
INRS-Institut Armand Frappier, Laval, Canada.
Proteomics. 2016 Dec;16(24):3183-3187. doi: 10.1002/pmic.201600290.
Plant cells are surrounded by cell walls playing many roles during development and in response to environmental constraints. Cell walls are mainly composed of polysaccharides (cellulose, hemicelluloses and pectins), but they also contain proteins which are critical players in cell wall remodeling processes. Today, the cell wall proteome of Arabidopsis thaliana, a major dicot model plant, comprises more than 700 proteins predicted to be secreted (cell wall proteins-CWPs) identified in different organs or in cell suspension cultures. However, the cell wall proteome of rosettes is poorly represented with only 148 CWPs identified after extraction by vacuum infiltration. This new study allows enlarging its coverage. A destructive method starting with the purification of cell walls has been performed and two experiments have been compared. They differ by the presence/absence of protein separation by a short 1D-electrophoresis run prior to tryptic digestion and different gradient programs for peptide separation before mass spectrometry analysis. Altogether, the rosette cell wall proteome has been significantly enlarged to 361 CWPs, among which 213 newly identified in rosettes and 57 newly described. The identified CWPs fall in four major functional classes: 26.1% proteins acting on polysaccharides, 11.1% oxido-reductases, 14.7% proteases and 11.7% proteins possibly related to lipid metabolism.
植物细胞被细胞壁所包围，细胞壁在植物发育过程以及应对环境限制时发挥着多种作用。细胞壁主要由多糖（纤维素、半纤维素和果胶）组成，但也含有蛋白质，这些蛋白质是细胞壁重塑过程中的关键参与者。如今，拟南芥（一种主要的双子叶模式植物）的细胞壁蛋白质组包含700多种预测会被分泌的蛋白质（细胞壁蛋白-CWPs），这些蛋白质已在不同器官或细胞悬浮培养物中得到鉴定。然而，莲座叶的细胞壁蛋白质组研究较少，通过真空渗透提取后仅鉴定出148种CWPs。这项新研究扩大了其覆盖范围。研究采用了一种从细胞壁纯化开始的破坏性方法，并比较了两个实验。这两个实验的不同之处在于，在胰蛋白酶消化之前是否通过短程一维电泳进行蛋白质分离，以及在质谱分析之前肽段分离所采用的不同梯度程序。总体而言，莲座叶细胞壁蛋白质组已显著扩大至361种CWPs，其中213种是在莲座叶中首次鉴定出来的，57种是新描述的。所鉴定的CWPs分为四大功能类别：作用于多糖的蛋白质占26.1%，氧化还原酶占11.1%，蛋白酶占14.7%，可能与脂质代谢相关的蛋白质占11.7%。
