Zewdie O, Mihret A, Ameni G, Worku A, Gemechu T, Abebe T
Department of Medical Laboratory Sciences, College of Medical and Health Sciences, Wollega University, Nekemte, Addis Ababa; Department of Microbiology, Immunology and Parasitology, College of Health Sciences, Addis Ababa University, Addis Ababa, Addis Ababa.
Department of Microbiology, Immunology and Parasitology, College of Health Sciences, Addis Ababa University, Addis Ababa, Addis Ababa; Armauer Hansen Research Institute, Addis Ababa, Addis Ababa.
Int J Tuberc Lung Dis. 2016 Nov;20(11):1529-1534. doi: 10.5588/ijtld.15.1023.
In Ethiopia, one of the world's 22 high tuberculosis (TB) burden countries, one third of the tuberculosis (TB) cases are attributed to tuberculosis lymphadenitis (TBLN). However, information on the molecular type of the mycobacterial species and strains that cause TBLN in the country is scarce.
To identify the species and strains of mycobacteria that cause TBLN in Ethiopia.
A cross-sectional study was conducted among 206 presumed TBLN cases to characterise positive culture isolates. Mycobacterium tuberculosis complex species and strains were identified using region of difference 9 deletion and SITVIT WEB, respectively. Of the 206 fine-needle aspirate samples collected, 74 (36%) were culture-positive: 73 (98.6%) of the isolates were M. tuberculosis, and the remaining 1.4% were M. bovis. Further characterisation of the 73 M. tuberculosis isolates led to 26 distinct spoligotype international types (SITs) and 13 newly identified patterns. The most prevalent strains were SIT149, SIT53, SIT26 and SIT37 of sublineages T3-ETH, T1, CASI-DELHI and T3, respectively; these accounted for 52.6% of the total number of strains.
TBLN was mainly caused by M. tuberculosis and highly clustered strains SIT149, SIT53, SIT26 and SIT37 of sublineages T3-ETH, T1, CASI-DELHI and T3, respectively.
埃塞俄比亚是世界上22个结核病高负担国家之一，三分之一的结核病病例归因于结核性淋巴结炎（TBLN）。然而，该国关于导致TBLN的分枝杆菌物种和菌株的分子类型的信息稀缺。
鉴定埃塞俄比亚导致TBLN的分枝杆菌物种和菌株。
对206例疑似TBLN病例进行横断面研究，以鉴定阳性培养分离株。分别使用差异区域9缺失和SITVIT WEB鉴定结核分枝杆菌复合群物种和菌株。在收集的206份细针抽吸样本中，74份（36%）培养呈阳性：73份（98.6%）分离株为结核分枝杆菌，其余1.4%为牛分枝杆菌。对73株结核分枝杆菌分离株的进一步鉴定导致26种不同的间隔寡核苷酸分型国际类型（SITs）和13种新鉴定出的模式。最常见的菌株分别是T3-ETH、T1、CASI-DELHI和T3亚系的SIT149、SIT53、SIT26和SIT37；这些菌株占菌株总数的52.6%。
TBLN主要由结核分枝杆菌引起，分别为T3-ETH、T1、CASI-DELHI和T3亚系的高度聚集菌株SIT149、SIT53、SIT26和SIT37。
