Li Hengye, Zhang Xuemeng, Zhang Lin, Wang Xiaojin, Kong Fenying, Fan Dahe, Li Lei, Wang Wei
School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, Jiangsu, 224000, China.
Yancheng Entry-Exit Inspection and Quarantine Bureau, Yancheng 224000, China.
J Chromatogr A. 2016 Nov 18;1473:90-98. doi: 10.1016/j.chroma.2016.10.050. Epub 2016 Oct 20.
In this study, a boronate affinity silica stationary phase with enhanced binding properties towards cis-diol compounds was prepared through the combination of surface-initiated atom transfer radical polymerization (SI-ATRP) with a Wulff-type boronate as affinity ligand. The stationary phase showed good hydrophilicity and improved binding strength toward adenosine, with binding constant to be as low as 2.38×10M. The column exhibited excellent binding specificity, low binding pH (≥5.5) and high binding capacities (80.1μmol adenosine g at pH 7.0 and 45.2μmol adenosine g at pH 5.5, respectively). The stationary phase was applied as adsorbent for the selective extraction of nucleosides in human urine with excellent specificity and high enrichment efficiency. These results demonstrated that this stationary phase could be favorably applied for selective capture and enrichment of cis-diol compounds in complex samples.
在本研究中，通过表面引发的原子转移自由基聚合（SI-ATRP）与作为亲和配体的乌尔曼型硼酸酯相结合，制备了一种对顺式二醇化合物具有增强结合性能的硼酸酯亲和硅胶固定相。该固定相对腺苷表现出良好的亲水性和提高的结合强度，结合常数低至2.38×10M。该柱表现出优异的结合特异性、低结合pH值（≥5.5）和高结合容量（在pH 7.0时为80.1μmol腺苷/g，在pH 5.5时为45.2μmol腺苷/g）。该固定相用作人尿中核苷选择性萃取的吸附剂，具有优异的特异性和高富集效率。这些结果表明，该固定相可有利地用于复杂样品中顺式二醇化合物的选择性捕获和富集。
