State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; Wuxi SensingNet Industrialization Research Institute, Wuxi 214000, China.
J Hazard Mater. 2017 Feb 5;323(Pt B):710-718. doi: 10.1016/j.jhazmat.2016.10.041. Epub 2016 Oct 19.
Inactivating antibiotic resistant bacteria (ARB) and removing antibiotic resistance genes (ARGs) are very important to prevent their spread into the environment. Previous efforts have been taken to eliminate ARB and ARGs from aqueous solution and sludges, however, few satisfying results have been obtained. This study investigated whether photocatalysis by TiO was able to reduce the two ARGs, mecA and ampC, within the host ARB, methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa, respectively. The addition of HO and matrix effect on the removal of ARB and ARGs were also studied. TiO thin films showed great effect on both ARB inactivation and ARGs removal. Approximately 4.5-5.0 and 5.5-5.8 log ARB reductions were achieved by TiO under 6 and 12mJ/cm UV fluence dose, respectively. For ARGs, 5.8 log mecA reduction and 4.7 log ampC reduction were achieved under 120mJ/cm UV fluence dose in the presence of TiO. Increasing dosage of HO enhanced the removal efficiencies of ARB and ARGs. The results also demonstrated that photocatalysis by TiO was capable of removing both intracellular and extracellular forms of ARGs. This study provided a potential alternative method for the removal of ARB and ARGs from aqueous solution.
灭活抗生素耐药菌（ARB）和去除抗生素耐药基因（ARGs）对于防止它们传播到环境中非常重要。之前已经采取了许多措施来从水溶液和污泥中去除 ARB 和 ARGs，但收效甚微。本研究探讨了 TiO 的光催化作用是否能够降低宿主 ARB 耐甲氧西林金黄色葡萄球菌（MRSA）和铜绿假单胞菌中的两种 ARGs，mecA 和 ampC。还研究了 HO 的添加和基质效应对 ARB 和 ARGs 去除的影响。TiO 薄膜对 ARB 失活和 ARGs 去除都有很大的影响。在 6 和 12mJ/cm 的 UV 光通量剂量下，TiO 分别实现了约 4.5-5.0 和 5.5-5.8 的对数 ARB 减少。在存在 TiO 的情况下，UV 光通量剂量为 120mJ/cm 时，mecA 的减少量达到了 5.8 个对数，ampC 的减少量达到了 4.7 个对数。HO 用量的增加提高了 ARB 和 ARGs 的去除效率。研究结果还表明，TiO 的光催化作用能够去除细胞内和细胞外形式的 ARGs。本研究为从水溶液中去除 ARB 和 ARGs 提供了一种潜在的替代方法。
